Practical Flow Cytometry

Practical Flow Cytometry

by Howard M. Shapiro
     
 

The fourth edition of this standard reference for analytical cytology using laser-activated detection and cell sorting covers technology, principles, and biomedical applications of flow cytometry. Shapiro updated the edition with a revised introduction; expanded coverage of applications for biotechnology, cell biology, genetics, hematology, immunology, microbiology… See more details below

Overview

The fourth edition of this standard reference for analytical cytology using laser-activated detection and cell sorting covers technology, principles, and biomedical applications of flow cytometry. Shapiro updated the edition with a revised introduction; expanded coverage of applications for biotechnology, cell biology, genetics, hematology, immunology, microbiology and oncology; discussions of new commercial instrumentation; alternatives to flow sorting; and updated references and suppliers list. The book is suggested for those working in immunology, cell and molecular biology, microbiology, oncology, or hematology. Annotation ©2003 Book News, Inc., Portland, OR

Product Details

ISBN-13:
9780845102435
Publisher:
Liss, Alan R. Inc.
Publication date:
02/01/1985
Pages:
314

Table of Contents

List of Tables and Figures
Preface to the Fourth Edition: Why You Should Read This Book - Or Not
Foreword to the Third Edition
Preface to the Third Edition
Preface to the Second Edition
Foreword to the First Edition
Preface to the First Edition
1Overture1
1.1What (And What Good) Is Flow Cytometry?1
1.2Beginnings: Microscopy and Cytometry2
1.3Problem Number One: Find the Cell(s)14
1.4Flow Cytometry: Problems, Parameters, Probes, and Principles18
1.5What's In the Box: Flow Cytometer Anatomy, Physiology, and Pathology49
1.6Alternatives to Flow Cytometry; Cytometer Ecology59
1.7The Rest of the Book60
2Learning Flow Cytometry61
2.1Information Souces and Resources62
2.2The Reader's Guide to Periodical Literature64
2.3Resources And Courses66
2.4Exploring The Foundations68
2.5Alternatives to Flow Cytometry71
3History73
3.1Ancient History73
3.2Classical History79
3.3Modern History85
4How Flow Cytometers Work101
4.1Light and Matter101
4.2Optical Systems119
4.3Light Sources124
4.4Light Collection149
4.5Detectors160
4.6Flow Systems166
4.7Electronic Measurements180
4.8Analog Signal Processing183
4.9Digital Signal Processing204
4.10Performance: Precision, Sensitivity, and Accuracy214
5Data Analysis225
5.1Goals and Methods in Data Analysis225
5.2Computer Systems for Flow Cytometry227
5.3Primary Data: Frequency Distributions231
5.4Compensating Without Decompensating242
5.5Dealing With the Data244
5.6Multiparameter Data Analysis248
5.7Analysis of Collected Data: How Much Is Enough / Too Much?253
5.8Data Analysis Odds and Ends254
6Flow Sorting257
6.1Sort Control (Decision) Logic257
6.2Preselected Count Circuits and Single Cell Sorting258
6.3Droplet Sorting, High-Speed and Low258
6.4Fluidic Switching Cell Sorters264
6.5Cell Manipulation By Optical Trapping266
6.6Cell Damage Cell Selection ("Cell Zapping")266
6.7Measures of Cell Sorter Performance: Purity, Recovery (Yield), and Efficiency267
6.8Other Considerations268
6.9Biohazard Control and Biosafety in Flow Cytometers and Sorters271
6.10Conclusions271
7Parameters and Probes271
7.1Physical Parameters and Their Uses273
7.2Intrinsic Cellular Parameters285
7.3Probes, Labels, and (Not) Protocols for Extrinsic Parameter Measurements293
7.4Nucleic Acid Dyes and Their Uses306
7.5Flourescent Labels and Protein Dyes326
7.6Improving Signals from Labels: Amplification and Other Techniques340
7.7Measuring Cell Surface and Intracellular Antigens345
7.8Nucleic Acid Sequence Detection361
7.9Probes for Various Cell Constituents362
7.10Time as a Parameter: Kinetic Measurements364
7.11Labeled Ligand Binding366
7.12Flunctional Parameters I369
7.13Functional Probes II: Indicators of Cell Activation381
7.14Reporter Genes408
8Buying Flow Cytometers411
8.1Introduction411
8.2History411
8.3BD Biosciences412
8.4Beckman Coulter, Inc418
8.5DakoCytomation423
8.6Cytopeia425
8.7Optoflow AS426
8.8Partec GmbH427
8.9Some Other Flow Cytometer Companies429
8.10Hematology Instruments, Etc.433
8.11Little Orphan Analyzers (And Big Orphan Sorters)434
8.12Third Party Software437
8.13The Selling of Flow Cytometers: Hype and Reality437
8.14Applying for a Grant for a Cytometer438
9Building Flow Cytometers441
9.1Why Buy a Flow Cytometer?441
9.2Why Build a Flow Cytometer?441
9.3Learning to Build Your Own442
10Using Flow Cytometers: Applications, Extensions, and Alternatives443
10.1The Daily Grind443
10.2Significant Events in the Lives of Cells448
10.3Identification of Cells in Mixed Populations464
10.4Tricks and Twists: Odd Jobs for Flow Cytometry471
10.5Single Cell Analysis: When Flow Won't Do475
10.6Applications of Flow Cytometry476
11Sources of Supply543
11.1Resources, Societies, Journals543
11.2Optical Supply Houses543
11.3Probes and Reagents544
11.4Calibration Particles / Cytometry Controls548
11.5Flow Cytometers549
11.6Data Analysis Software / Systems551
11.7Cytometer Rehabilitation / Add-ons553
11.8Flow Cytometer Parts553
11.9Lasers555
11.10Optical Filters556
11.11Aids to Troubleshooting Flow Cytometers When All Else Fails557
11.12Proficiency Testing557
11.13Sex Selection557
11.14Alternative Technology558
12Afterword561
12.1Dotting i's and Crossing t's561
12.2Late Breaking News561
12.3Analytical Biology, Such as it Isn't: Is This Any Way to Run a Science?563
12.4Colophon564
12.5Unfinished Business565
12.6Flow and the Human Condition566
12.7One More Thing566

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