Practical Flow Cytometry / Edition 2

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Practical Flow Cytometry provides the most readable and comprehensive resource covering the technology principles, and biomedical applications of analytical cytology using laser activated detection and cell sorting. The book is required reading for anyone in immunology, cell and molecular biology, microbiology, oncology or hematology.

The book contains black-and-white illustrations.

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Editorial Reviews

T. Vincent Shankey
This is a comprehensive treatment of flow cytometry and the diverse technologies that are used to prepare and analyze samples for analysis using flow cytometry. The information provided is useful to understand how to effectively use flow cytometry, including what went wrong and how to fix it, how to analyze the data generated, and how to improve the whole lot. The purpose is to ""include almost anything anyone might want, or need to know about flow cytometry."" With the continuing improvements to the technologies and increasing areas of application of flow cytometry, the current revision (currently in its third edition) represents a timely and needed expansion of earlier editions. This book is targeted at anyone who uses or wishes to use a flow cytometer. Although the treatment of all areas begins with fundamental principles, current areas of application and new technologies and applications are included, making the book of interest to beginners and experts alike. The presentation is uniform and the book is easy to read. Although the diversity of subject places significant demands on a single author, Dr. Shapiro is unique in this field in his comprehension of all these areas. The book includes a wealth of practical information, including a listing other books published on any aspect of flow cytometry, societies involved with this technology, and a listing of instrument and reagent sources. This book is an encyclopedia of flow cytometry. The only criticism might be that it covers every aspect of flow cytometry in considerable detail, sometimes more detail than a reader pushed by many demands wants (including why the flow cytometry experiment did not work). Most flow cytometrylaboratories already have previous editions placed near the cytometer, and the more sophisticated or advanced the laboratory, the more battered and used the book. With the improvements and expansion in this edition, it will likely sit next to previous and well-worn editions in most flow cytometry laboratories (including mine).
From the Publisher
"one of the most valuable of its genre and...addressed to a wide audience.... Written in such an attractive way, being both informative and stimulating" (Zbigniew Darzynkiewicz, Trends in Cell Biology)

"...there is no question that this is THE reference book on flow cytometry...You need this book if you use flow cytometry. Get it!" (Doody's Electronic Journal, October 25, 2003)

"...This complete reference examines the vast biomedical applications-fully updated and revised..." (Jnl. Of Investigational Allergology and Clinical Immunology, vol 13; no 4)

Doody's Review Service
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This is a truly comprehensive book on everything you could ever want to know about flow cytometry.
Purpose: The purpose of this book is to be THE reference book on flow cytometry. This is the fourth edition of this book, and there is no question that it is THE reference book on flow cytometry!
Audience: This book would be useful to anyone who uses flow cytometry or relies on this technology for their research or clinical care.
Features: This book continues in its grand tradition of being the "bible" of flow cytometry. I cannot adequately describe its depth and breadth, so I resort to sheer astounding numbers to portray its comprehensiveness - 566 pages of 10 point font, surprisingly few illustrations (yes, it's primarily text), 3,685(!) references. In briefly skimming the book, I was overwhelmed by the massive amount of information. No question that I'll have this book within easy reach whenever I field questions about flow cytometry! A really nice touch in this book is the author's chatty (and sometimes quirky) writing style. The reader will not only find accurate and complete factual information, but will also appreciate flow cytometry-themed lyrics to sing to the tune for famous melodies, Shapiro's various (and amusing) laws, and helpful tips (including the final one that warns the user to never use a staff to smite a clogged cytometer). The author's humor and conversational style makes interesting reading and the information incredibly accessible. A major difference between this and the third edition is the elimination of the section on the "do-it-yourself" flow cytometer (e.g., "cytomutt") - the author's acknowledgement of the widespread penetration of commercial systems in the field. In this regard, there is a very helpful chapter listing the various manufacturers and companies from which reagents can be purchased. Finally - and rare in these types of comprehensive books - there is touching recognition to those who were pioneers in this field. A really nice human touch to a very complex subject.
Assessment: Wow! You need this book if you use flow cytometry. Get it!

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780845142547
  • Publisher: Liss, Alan R. Inc.
  • Publication date: 1/1/1988
  • Edition number: 2
  • Pages: 353

Table of Contents

List of Tables and Figures
Preface to the Fourth Edition: Why You Should Read This Book - Or Not
Foreword to the Third Edition
Preface to the Third Edition
Preface to the Second Edition
Foreword to the First Edition
Preface to the First Edition
1 Overture 1
1.1 What (And What Good) Is Flow Cytometry? 1
1.2 Beginnings: Microscopy and Cytometry 2
1.3 Problem Number One: Find the Cell(s) 14
1.4 Flow Cytometry: Problems, Parameters, Probes, and Principles 18
1.5 What's In the Box: Flow Cytometer Anatomy, Physiology, and Pathology 49
1.6 Alternatives to Flow Cytometry; Cytometer Ecology 59
1.7 The Rest of the Book 60
2 Learning Flow Cytometry 61
2.1 Information Souces and Resources 62
2.2 The Reader's Guide to Periodical Literature 64
2.3 Resources And Courses 66
2.4 Exploring The Foundations 68
2.5 Alternatives to Flow Cytometry 71
3 History 73
3.1 Ancient History 73
3.2 Classical History 79
3.3 Modern History 85
4 How Flow Cytometers Work 101
4.1 Light and Matter 101
4.2 Optical Systems 119
4.3 Light Sources 124
4.4 Light Collection 149
4.5 Detectors 160
4.6 Flow Systems 166
4.7 Electronic Measurements 180
4.8 Analog Signal Processing 183
4.9 Digital Signal Processing 204
4.10 Performance: Precision, Sensitivity, and Accuracy 214
5 Data Analysis 225
5.1 Goals and Methods in Data Analysis 225
5.2 Computer Systems for Flow Cytometry 227
5.3 Primary Data: Frequency Distributions 231
5.4 Compensating Without Decompensating 242
5.5 Dealing With the Data 244
5.6 Multiparameter Data Analysis 248
5.7 Analysis of Collected Data: How Much Is Enough / Too Much? 253
5.8 Data Analysis Odds and Ends 254
6 Flow Sorting 257
6.1 Sort Control (Decision) Logic 257
6.2 Preselected Count Circuits and Single Cell Sorting 258
6.3 Droplet Sorting, High-Speed and Low 258
6.4 Fluidic Switching Cell Sorters 264
6.5 Cell Manipulation By Optical Trapping 266
6.6 Cell Damage Cell Selection ("Cell Zapping") 266
6.7 Measures of Cell Sorter Performance: Purity, Recovery (Yield), and Efficiency 267
6.8 Other Considerations 268
6.9 Biohazard Control and Biosafety in Flow Cytometers and Sorters 271
6.10 Conclusions 271
7 Parameters and Probes 271
7.1 Physical Parameters and Their Uses 273
7.2 Intrinsic Cellular Parameters 285
7.3 Probes, Labels, and (Not) Protocols for Extrinsic Parameter Measurements 293
7.4 Nucleic Acid Dyes and Their Uses 306
7.5 Flourescent Labels and Protein Dyes 326
7.6 Improving Signals from Labels: Amplification and Other Techniques 340
7.7 Measuring Cell Surface and Intracellular Antigens 345
7.8 Nucleic Acid Sequence Detection 361
7.9 Probes for Various Cell Constituents 362
7.10 Time as a Parameter: Kinetic Measurements 364
7.11 Labeled Ligand Binding 366
7.12 Flunctional Parameters I 369
7.13 Functional Probes II: Indicators of Cell Activation 381
7.14 Reporter Genes 408
8 Buying Flow Cytometers 411
8.1 Introduction 411
8.2 History 411
8.3 BD Biosciences 412
8.4 Beckman Coulter, Inc 418
8.5 DakoCytomation 423
8.6 Cytopeia 425
8.7 Optoflow AS 426
8.8 Partec GmbH 427
8.9 Some Other Flow Cytometer Companies 429
8.10 Hematology Instruments, Etc. 433
8.11 Little Orphan Analyzers (And Big Orphan Sorters) 434
8.12 Third Party Software 437
8.13 The Selling of Flow Cytometers: Hype and Reality 437
8.14 Applying for a Grant for a Cytometer 438
9 Building Flow Cytometers 441
9.1 Why Buy a Flow Cytometer? 441
9.2 Why Build a Flow Cytometer? 441
9.3 Learning to Build Your Own 442
10 Using Flow Cytometers: Applications, Extensions, and Alternatives 443
10.1 The Daily Grind 443
10.2 Significant Events in the Lives of Cells 448
10.3 Identification of Cells in Mixed Populations 464
10.4 Tricks and Twists: Odd Jobs for Flow Cytometry 471
10.5 Single Cell Analysis: When Flow Won't Do 475
10.6 Applications of Flow Cytometry 476
11 Sources of Supply 543
11.1 Resources, Societies, Journals 543
11.2 Optical Supply Houses 543
11.3 Probes and Reagents 544
11.4 Calibration Particles / Cytometry Controls 548
11.5 Flow Cytometers 549
11.6 Data Analysis Software / Systems 551
11.7 Cytometer Rehabilitation / Add-ons 553
11.8 Flow Cytometer Parts 553
11.9 Lasers 555
11.10 Optical Filters 556
11.11 Aids to Troubleshooting Flow Cytometers When All Else Fails 557
11.12 Proficiency Testing 557
11.13 Sex Selection 557
11.14 Alternative Technology 558
12 Afterword 561
12.1 Dotting i's and Crossing t's 561
12.2 Late Breaking News 561
12.3 Analytical Biology, Such as it Isn't: Is This Any Way to Run a Science? 563
12.4 Colophon 564
12.5 Unfinished Business 565
12.6 Flow and the Human Condition 566
12.7 One More Thing 566
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