Practical Flow Cytometry in Haematology Diagnosis

Practical Flow Cytometry in Haematology Diagnosis

by Mike Leach, Mark Drummond, Allyson Doig
     
 

View All Available Formats & Editions

Practical Flow Cytometry in Haematology Diagnosis Dr Mike Leach, Dr Mark Drummond and Dr Allyson Doig Department of Haematology, Gartnavel General Hospital, Glasgow, UK Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UK Department of Haematology, Gartnavel Hospital, Glasgow, UK The accurate diagnosis of haematologic malignancies is a

See more details below

Overview

Practical Flow Cytometry in Haematology Diagnosis Dr Mike Leach, Dr Mark Drummond and Dr Allyson Doig Department of Haematology, Gartnavel General Hospital, Glasgow, UK Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UK Department of Haematology, Gartnavel Hospital, Glasgow, UK The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: • A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditions • Carefully chosen real-life cases in each chapter, complemented by high quality morphological images • Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide. Titles of related interest Ortolani: Flow Cytometry of Hematological Malignancies (2011) ISBN: 978-1-4443-3588-0 Kottke-Marchant: Laboratory Hematology Practice (2012) ISBN: 978-1-4051-6218-0

Read More

Editorial Reviews

From the Publisher

“I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory.”  (British Journal of Haematology, 14 November 2014)

“There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees.”  (Haem Trainee, 1 January 2014)

“This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.”  (Doody’s, 19 July 2013)

Doody's Review Service
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: True to its title, this is a practical book on the use of flow cytometry in hematology/hematopathology diagnosis.
Purpose: The purpose is to provide a practical guide to uses of flow cytometry, following the typical presentation and thought process commonly undertaken clinically.
Audience: Practicing hematologists/oncologists reliant on flow cytometry results for diagnosis of disease and management are the intended audience. This book also would be useful for hematopathologists, pathology residents, hematopathology or hematology/oncology fellows and clinical laboratory scientists (CLSs) performing flow cytometry analysis.
Features: This book details a very practical approach to the use of flow cytometry in the diagnosis of hematological or hematopathological disease. It effectively uses a case-based method ("worked example") to correlate and integrate clinical symptoms, morphology, and flow cytometry findings and arrive at the correct diagnosis. It is clear, concise, and quite accessible. I really liked table 5.5 comparing and contrasting WHO 2008 and FAB AML diagnoses, typical antigen expression, notable negative antigens, and notable and aberrant antigens. All concisely in one table - wow! I also liked that the book covers normal, reactive and non-neoplastic hematological disorders. One very minor gripe: the color reproduction of the Wright-Giemsa stains is not perfect. Many of the photomicrographs have a bluish background tint; eosinophilic granules in worked example 5.2 appear blue. One bigger gripe: there is a very brief discussion of flow cytometry testing of bronchoalveolar lavage fluid, yet there is no discussion of safety precautions necessary to prevent transmission of aerosol transmissible infectious agents (e.g., Mycobacterium tuberculosis).
Assessment: This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.

Read More

Product Details

ISBN-13:
9780470671207
Publisher:
Wiley
Publication date:
04/29/2013
Pages:
256
Product dimensions:
7.50(w) x 9.70(h) x 0.70(d)

Related Subjects

Read an Excerpt

Click to read or download

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >