A Practical Guide to the Study of Calcium in Living Cells / Edition 1

A Practical Guide to the Study of Calcium in Living Cells / Edition 1

by Leslie Wilson
     
 

ISBN-10: 0125228104

ISBN-13: 9780125228107

Pub. Date: 03/10/1994

Publisher: Elsevier Science

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal

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Overview

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+ buffers appropriate to their research needs.
Highlights
• Describes techniques to
• Detect Ca2+ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
• Perturb intracellular Ca2+
• Prepare Ca2+ buffers with an easy-to-use computer program

• Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
• Covers methods in detail and describes the problems and pitfalls of each technique

Product Details

ISBN-13:
9780125228107
Publisher:
Elsevier Science
Publication date:
03/10/1994
Series:
Methods in Cell Biology Series
Edition description:
SPIRAL
Pages:
368
Product dimensions:
6.00(w) x 9.00(h) x (d)

Table of Contents

Contributors
Preface
1A Practical Guide to the Preparation of Ca[superscript 2+] Buffers
2Photorelease Techniques for Raising or Lowering Intracellular Ca[superscript 2+]
3Pharmacological Tools for Perturbing Intracellular Calcium Storage
4How to Make and to Use Calcium-Specific Mini- and Microelectrodes
5The Vibrating Ca[superscript 2+] Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca[superscript 2+] Influx and Efflux
6Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels
7Practical Aspects of Measuring [Ca[superscript 2+]] with Fluorescent Indicators
8Rapid Simultaneous Estimation of Intracellular Calcium and pH
9Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques
10Confocal Imaging of Ca[superscript 2+] in Cells
11Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes
12Inexpensive Techniques for Measuring [Ca[superscript 2+]][subscript i] Changes Using a Photomultiplier Tube
13Imaging Ca[superscript 2+][subscript i] with Aequorin Using a Photon Imaging Detector
14Targeting Recombinant Aequorin to Specific Intracellular Organelles
Index
Contents of Previous Volumes

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