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A Practical Guide to the Study of Calcium in Living Cells / Edition 1
     

A Practical Guide to the Study of Calcium in Living Cells / Edition 1

by Leslie Wilson, Paul T. Matsudaira
 

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ISBN-10: 0125228104

ISBN-13: 9780125228107

Pub. Date: 03/10/1994

Publisher: Elsevier Science

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal

Overview

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+ buffers appropriate to their research needs.
Highlights
• Describes techniques to
• Detect Ca2+ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
• Perturb intracellular Ca2+
• Prepare Ca2+ buffers with an easy-to-use computer program

• Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
• Covers methods in detail and describes the problems and pitfalls of each technique

Product Details

ISBN-13:
9780125228107
Publisher:
Elsevier Science
Publication date:
03/10/1994
Series:
Methods in Cell Biology Series
Edition description:
SPIRAL
Pages:
368
Product dimensions:
6.00(w) x 9.00(h) x (d)

Table of Contents

Ca2+ Buffers and [Ca2+]I Perturbation Techniques:
D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers.
R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+.
D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes:
S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux.
C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
Fluorescence Techniques for Imaging [Ca2+]I:
J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators.
S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells.
S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.
Use of Aequorin for [Ca2+]I Imaging:
K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube.
A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector.
R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.
Index.

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