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Methodologies and databases for biochemistry and molecular biology are included in this easy-to-use laboratory reference. Its logical presentation enables the reader to quickly and conveniently locate the information relevant to his or her needs. Featured are tables containing data on amino acids, proteins, nucleosides, nucleotides, and nucleic acids. Also featured are lipids and physical chemical data.
Edited by a leading professional in the field, this compact, yet comprehensive bench manual serves as the definitive reference source for your laboratory.
AMINO ACIDS AND PROTEINS. AMINO ACIDS. Data on the Naturally Occurring Amino Acids. a, b-Unsaturated Amino Acids. Amino Acid Antagonists. Properties of the a-Keto Acid Analogs of Amino Acids. Far Ultraviolet Absorption Spectra of Amino Acids. UV Absorption Characteristics of N-Acetyl —Methyl Esters of the Aromatic Amino Acids, Cystine, and of N-Acetylcysteine. Numerical Values of the Absorbances of the Aromatic Amino Acids in Acid, Neutral, and Alkaline Solutions. Luminescence. Specific Rotatory Dispersion Constants for 0.1 M Amino Solutions. Amino Acid Suffixes and Prefixes. Poly (a-Amino) Acids. Poly (a-Amino) Acids, Their Solubility and Susceptibility to Enzymatic Activities. PROTEINS. Specificity of Reagents Commonly Used to Chemically Modify Proteins. Acid Hydrolysis of Proteins. Hydrolysis of Proteins. Index to Physical-Chemical Data of Proteins. The Proteins of Blood Coagulation. Glycoproteins. Characterization of Histones. Molecular Parameters of the Contractile Proteins. Subunit Constitution of Proteins. Molar Absorptivity and A11%cm Values for Proteins at Selected Wavelengths of the Ultraviolet and Visible Region. Protein pK Values. GEL ELECTROPHORESIS OF PROTEINS. SDS-Electrophoresis. Molecular Weight Analysis of Oligopeptides by Electrophoresis in Polyacrylamide Gel with Sodium Dodecyl Sulfate. Use of Dimethyl Suberimidate, a Cross-Linking Reagent, in Studying the Subunit Structure of Oligomeric Proteins. Prestained Electrophoresis Standards. Polyacrylamide Gels. Detection Methods for Enzymes After Separation in Starch or Acrylamide Gel. Starch Gels. NUCLEOSIDES, NUCLEOTIDES, AND NUCLEIC ACIDS. ALKYL BASES, NUCLEOSIDES AND NUCLEOTIDES. UV Spectra Characteristics and Acid Dissociation Constants of 280 Alkyl Bases, Nucleosides, and Nucleotides. UV Absorbance of Oligonucleotides Containing 2'-O-Methylpentose Residues. Optical Rotatory Dispersion Parameters of Oligonucleotides of RNA. Circular Dichroism Parameters of Oligonucleotides of RNA. Circular Dichroism Parameters of Oligonucleotides of DNA. FUNCTIONAL CHARACTERIZATIONS OF NUCLEOPHOSPHODIESTERASES. Table 1-Deoxyribonucleases. Table 2-Ribonuclease. Table 3-Nucleases Nonspecific for the Sugar Moiety. DEOXYNUCLEOTIDE POLYMERIZING ENZYMES. RIBONUCLEOTIDE POLYMERIZING ENZYMES. GEL ELECTROPHORESIS OF NUCLEIC ACIDS. Table 1-Two-Dimensional Gel Systems for RNA Separation. Table 2-Conditions for the Electrophoresis of Oligonucleotides: Comparison of the Original Procedure and Some Modifications. Table 3-Conditions for the Electrophoresis of RNA Fragments. Table 4-Conditions for the Electrophoresis of Small RNA Molecules. Table 5-Conditions for the Electrophoresis of mRNA. Table 6-Conditions for the Electrophoresis of DNA Restriction Fragments on Conventional Gels. Table 7-Conditions for the Electrophoresis of DNA Restriction Fragments on Denaturing Gradient Gels. References. ELECTROPHORESIS OF NUCLEOPROTEINS. Recipes of Gel Mixtures Used for Separating Polysomes and Ribosomes. NUCLEIC ACID MOLECULAR WEIGHT MARKERS. DNA Size Markers. RNA Size Markers. References. NUCLEASES. Endonucleases Specific for Single-Stranded Polynucleotides. Exodeoxyribonucleases of Escherichia coli. RecBC-Like Enzymes. Restriction and Modification Enzymes and Their Recognition Sequences. LIPIDS. FATTY ACIDS: PHYSICAL AND CHEMICAL CHARACTERISTICS. DIELECTRIC CONSTANTS OF SOME FATS, FATTY ACIDS, AND ESTERS. SOLUBILITIES OF FATTY ACIDS IN WATER. APPROXIMATE SOLUBILITIES OF WATER IN SATURATED FATTY ACIDS AT VARIOUS TEMPERATURES. SOLUBILITY OF SIMPLE SATURATED TRIGLYCERIDES. SOLUBILITIES OF MIXED TRIACID TRIGLYCERIDES AT 25° C. PROPERTIES AND FATTY ACID COMPOSITION OF FATS AND OILS. PHYSICAL-CHEMICAL DATA. THERMODYNAMIC TERMS RECOMMENDED. DECI-NORMAL SOLUTIONS OF OXIDATION AND REDUCTION REAGENTS. MEASUREMENT OF pH. Buffer Solutions. Amine Buffers Useful for Biological Research. Preparation of Buffers for Use in Enzyme Studies. Indicators for Volumetric Work and pH Determinations. Mixed Indicators. Concentration of Acids and Bases. TRANSMISSION LIMITS OF COMMON IONS. SPECTROPHOTOMETRIC DETERMINATION OF PROTEIN CONCENTRATION IN THE SHORT-WAVELENGTH ULTRAVIOLET. WEIGHTS OF CELLS AND CELL CONSTITUENTS. PARTICLE DIAMETER. CHEMICAL AND PHYSICAL PROPERTIES OF VARIOUS COMMERCIAL PLASTICS. CHEMICAL RESISTANCE AND OTHER PROPERTIES OF CENTERPIECE MATERIALS USED IN THE BECKMANâ MODEL-E ANALYTICAL ULTRACENTRIFUGE. CHEMICAL COMPATIBILITY OF MILLIPOREâ FILTERS, O-RINGS, AND FILTER HOLDERS. INDEX.