Practical HPLC Method Development / Edition 2

Practical HPLC Method Development / Edition 2

5.0 1
by Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch
     
 

ISBN-10: 047100703X

ISBN-13: 9780471007036

Pub. Date: 03/28/1997

Publisher: Wiley

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical

…  See more details below

Overview

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Product Details

ISBN-13:
9780471007036
Publisher:
Wiley
Publication date:
03/28/1997
Edition description:
REV
Pages:
800
Sales rank:
806,137
Product dimensions:
6.36(w) x 9.25(h) x 1.87(d)

Table of Contents

Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >

Practical HPLC Method Development 5 out of 5 based on 0 ratings. 1 reviews.
Guest More than 1 year ago
I have been performing HPLC assays for 3 years now and a few months ago was asked to help develop some new methods. Not knowing where to begin, I found this book, read the summary and took a chance on it: it payed off. This book covers everything one needs to know from the inital stages of development through the validation procedures. I thought I knew a lot about HPLC before I used this book, but I easily learned that much over again. A great resource for anyone developing HPLC assays.