Practical HPLC Method Development / Edition 2

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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

The book contains black-and-white illustrations.

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Editorial Reviews

Details current knowledge on method development for high-performance liquid chromatography HPLC. This second edition offers increased focus on specialized applications for separating analytes such as biological samples and mixtures of enantiomers, and expanded material on computer-assisted method development, HPLC column technology, and biochemical and chiral applications, integrating background and practical material from Snyder and Kirkland's "Introduction to Modern Liquid Chromatography". For scientists and students beginning work with HPLC as well as experienced analysts. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780471007036
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 3/28/1997
  • Edition description: REV
  • Edition number: 2
  • Pages: 800
  • Product dimensions: 6.36 (w) x 9.25 (h) x 1.87 (d)

Table of Contents

Glossary of Symbols and Terms
1 Getting Started 1
2 Basics of Separation 21
3 Detection Sensitivity and Selectivity 59
4 Sample Preparation 100
5 The Column 174
6 Non-ionic Samples: Reversed- and Normal-Phase HPLC 233
7 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC 292
8 Gradient Elution 350
9 Systematic Approach to the Reversed-Phase Separation of Regular Samples 402
10 Computer-Assisted Method Development 439
11 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds 479
12 Chiral Separations 537
13 Preparative HPLC Separation 616
14 Quantitation (Including Trace Analysis) 643
15 Completing the Method: Validation and Transfer 685
App. I Plate Number and Resolution 714
App. II Properties of Solvents Used in HPLC 721
App. III Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure 729
App. IV Preparing Buffered Mobile Phases 735
App. V Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers 740
App. VI Adjusting Mobile-Phase Water Content for Normal-Phase HPLC 744
Index 747
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  • Anonymous

    Posted February 21, 2001

    Great resource for everyone, beginners through experts

    I have been performing HPLC assays for 3 years now and a few months ago was asked to help develop some new methods. Not knowing where to begin, I found this book, read the summary and took a chance on it: it payed off. This book covers everything one needs to know from the inital stages of development through the validation procedures. I thought I knew a lot about HPLC before I used this book, but I easily learned that much over again. A great resource for anyone developing HPLC assays.

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