Practical HPLC Method Development / Edition 2

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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

The book contains black-and-white illustrations.

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Editorial Reviews

Details current knowledge on method development for high-performance liquid chromatography HPLC. This second edition offers increased focus on specialized applications for separating analytes such as biological samples and mixtures of enantiomers, and expanded material on computer-assisted method development, HPLC column technology, and biochemical and chiral applications, integrating background and practical material from Snyder and Kirkland's "Introduction to Modern Liquid Chromatography". For scientists and students beginning work with HPLC as well as experienced analysts. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780471007036
  • Publisher: Wiley
  • Publication date: 3/28/1997
  • Edition description: REV
  • Edition number: 2
  • Pages: 800
  • Sales rank: 399,726
  • Product dimensions: 6.36 (w) x 9.25 (h) x 1.87 (d)

Table of Contents

Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.



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Customer Reviews

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  • Anonymous

    Posted February 21, 2001

    Great resource for everyone, beginners through experts

    I have been performing HPLC assays for 3 years now and a few months ago was asked to help develop some new methods. Not knowing where to begin, I found this book, read the summary and took a chance on it: it payed off. This book covers everything one needs to know from the inital stages of development through the validation procedures. I thought I knew a lot about HPLC before I used this book, but I easily learned that much over again. A great resource for anyone developing HPLC assays.

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