Practical HPLC Method Development / Edition 2

Hardcover (Print)
Buy Used
Buy Used from BN.com
$116.47
(Save 41%)
Item is in good condition but packaging may have signs of shelf wear/aging or torn packaging.
Condition: Used – Good details
Used and New from Other Sellers
Used and New from Other Sellers
from $146.14
Usually ships in 1-2 business days
(Save 26%)
Other sellers (Hardcover)
  • All (12) from $146.14   
  • New (10) from $152.93   
  • Used (2) from $146.14   

Overview

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

The book contains black-and-white illustrations.

Read More Show Less

Editorial Reviews

Booknews
Details current knowledge on method development for high-performance liquid chromatography HPLC. This second edition offers increased focus on specialized applications for separating analytes such as biological samples and mixtures of enantiomers, and expanded material on computer-assisted method development, HPLC column technology, and biochemical and chiral applications, integrating background and practical material from Snyder and Kirkland's "Introduction to Modern Liquid Chromatography". For scientists and students beginning work with HPLC as well as experienced analysts. Annotation c. by Book News, Inc., Portland, Or.
Read More Show Less

Product Details

  • ISBN-13: 9780471007036
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 3/28/1997
  • Edition description: REV
  • Edition number: 2
  • Pages: 800
  • Product dimensions: 6.36 (w) x 9.25 (h) x 1.87 (d)

Table of Contents

Preface
Glossary of Symbols and Terms
1 Getting Started 1
2 Basics of Separation 21
3 Detection Sensitivity and Selectivity 59
4 Sample Preparation 100
5 The Column 174
6 Non-ionic Samples: Reversed- and Normal-Phase HPLC 233
7 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC 292
8 Gradient Elution 350
9 Systematic Approach to the Reversed-Phase Separation of Regular Samples 402
10 Computer-Assisted Method Development 439
11 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds 479
12 Chiral Separations 537
13 Preparative HPLC Separation 616
14 Quantitation (Including Trace Analysis) 643
15 Completing the Method: Validation and Transfer 685
App. I Plate Number and Resolution 714
App. II Properties of Solvents Used in HPLC 721
App. III Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure 729
App. IV Preparing Buffered Mobile Phases 735
App. V Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers 740
App. VI Adjusting Mobile-Phase Water Content for Normal-Phase HPLC 744
Index 747
Read More Show Less

Customer Reviews

Average Rating 5
( 1 )
Rating Distribution

5 Star

(1)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously
Sort by: Showing 1 Customer Reviews
  • Anonymous

    Posted February 21, 2001

    Great resource for everyone, beginners through experts

    I have been performing HPLC assays for 3 years now and a few months ago was asked to help develop some new methods. Not knowing where to begin, I found this book, read the summary and took a chance on it: it payed off. This book covers everything one needs to know from the inital stages of development through the validation procedures. I thought I knew a lot about HPLC before I used this book, but I easily learned that much over again. A great resource for anyone developing HPLC assays.

    Was this review helpful? Yes  No   Report this review
Sort by: Showing 1 Customer Reviews

If you find inappropriate content, please report it to Barnes & Noble
Why is this product inappropriate?
Comments (optional)