Practical Laboratory Andrology / Edition 1

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Overview

This practical manual on sperm analysis presents the diagnostic and therapeutic procedures that are used in andrology laboratories to analyze and assess male infertility. Diagnostic areas include: semen analysis and the biochemical, immunological and microbiological examination of human semen and spermatozoa; computer-aided sperm motility analysis; sperm ultrastructure; and assessment of sperm transport through the female tract and sperm fertilizing ability. The clinical relevance of various diagnostic procedures is also discussed. Therapeutic topics include sperm washing techniques, semen cryopreservation, and insemination procedures. The volume also covers safety in the andrology laboratory, technician training, and quality control. The text is extensively illustrated and will be an invaluable resource to all scientists and technicians who diagnose male infertility. It will also be of interest to researchers working in human gamete biology and reproductive physiology. The detailed methods described in the book are relevant to all hospital, commercial, and university laboratories involved in infertility diagnosis and treatment.

This book contains black-and-white illustrations.

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Product Details

  • ISBN-13: 9780195065954
  • Publisher: Oxford University Press, USA
  • Publication date: 1/28/1994
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 416
  • Product dimensions: 6.38 (w) x 9.50 (h) x 1.02 (d)

Table of Contents

1 Introduction 3
2 Sperm Physiology 13
3 Semen Analysis 43
4 Biochemistry of Spermatozoa and Seminal Plasma 89
5 Antisperm Antibodies 111
6 Semen Microbiology and Virology 127
7 Computer-Aided Sperm Analysis and Sperm Kinematics 135
8 Ultrastructure of Spermatozoa 159
9 Assessment of Sperm Transport 175
10 Sperm Fertilizing Ability Testing 199
11 Clinical Relevance of Diagnostic Procedures 241
12 Sperm Washing 267
13 Therapeutic Insemination Procedures 287
14 Semen Cryopreservation 301
15 Safety in the Andrology Laboratory 327
16 Technician Training and Quality Control Aspects 337
App. I Semen Analysis Procedural Schedule and Sample Laboratory Form 349
App. II Microscope Field Calibration 352
App. III Epifluorescence 356
App. IV Sample Antisperm Antibody Laboratory Report Form 359
App. V Sample Semen Donor Screening Forms 361
App. VI Sample Sperm-Mucus Interaction Testing Laboratory and Report Forms 364
App. VII Semen Cryopreservation Forms 371
App. VIII Equipment Required for a Basic Andrology Laboratory 376
Index 381
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