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“This excellent book is a must for all biology departments...tells you all you ever wanted to know about practical techniques but didn’t like to ask”
Journal of Biological Education
“There is something here for everyone, including advanced level students and teachers”
“A well-pitched and comprehensive guide for all life science undergraduate students; in many cases this could be the most important book that they will buy.”
Dr. Chris Bax, London Metropolitan University
If you are studying Biology then this book is an indispensable companion throughout your entire degree programme. It lucidly demonstrates the laboratory and field skills that you will draw on time and again for the practical aspects of your studies, and also gives you a solid grounding in those wider transferable skills which are increasingly necessary to achieve a higher level of academic success.
This fourth edition builds on the excellent foundation of its predecessors. It contains a fully updated examination of existing topics and covers even more core skills, including:
Allan Jones is Senior Lecturer in in Biology and Life Sciences at the University of Dundee; Rob Reed is Professor of Biomedical Sciences at Central Queensland University; Jonathan Weyers is Director of Quality Assurance at the University of Dundee.
1. Basic Principles.
2. Health and Safety.
3. Working with Liquids.
4. Basic Laboratory Procedures.
5. Principles of Solution Chemistry.
6. pH and Buffer Solutions.
II. THE INVESTIGATIVE APPROACH.
7. The Principles of Measurement.
8. SI Units and their Use.
9. Making Observations.
10. Drawings and Diagrams.
11. Samples and Sampling.
12. Scientific Method and Design of Experiments.
13. Making Notes of Practical Work.
14. Project Work.
III. OBTAINING AND IDENTIFYING SPECIMENS.
15. Collating Animals and Plants.
16. Fixing and Preserving Animals and Plants.
17. Collating and Isolating Microbes.
18. Naming and Classifying Organisms.
19. Identifying Plants and Animals.
20. Identifying Microbes.
IV. MANIPULATING AND OBSERVING SPECIMENS.
21. The Purpose and Practice of Dissection.
22. Introduction to Microscopy.
23. Preparing Slides for Light Microscopy.
24. Setting Up and Using a Light Microscope.
25. Interpreting Microscope Images.
26. Sterile Technique.
27. Cell Culture.
28. Working with Animal and Plant Tissues and Cells.
29. Photography and Imaging.
30. Measuring Growth and Response.
V. ADVANCED LABORATORY TECHNIQUES.
31. Immunological Methods.
32. Enzyme Studies.
33. Mendelian Genetics.
34. Genetic Engineering Techniques.
35. Light Measurement.
36. Radioactive Isotopes and their Uses.
37. Measuring Oxygen Content.
39. Spectroscopy Techniques.
VI. ANALYSIS AND PRESENTATION OF DATA.
41. Manipulating and Transforming Raw Data.
42. Using Graphs.
43. Presenting Data inTables.
44. Hints for Solving Numerical Problems.
45. Descriptive Statistics.
46. Choosing and Using Statistical Tests.
VII. INFORMATION TECHNOLOGY AND LIBRARY RESOURCES.
47. The Internet and the World Wide Web.
48. Using Spreadsheets.
49. Word Processors, Databases, and Other Packages.
50. Finding and Citing Published Information.
VIII. COMMUNICATING INFORMATION.
51. General Aspects of Scientific Writing.
52. Writing Essays.
53. Reporting Practical and Project Work.
54. Writing Literature Surveys and Reviews.
55. Organizing a Poster Display.
56. Giving an Oral Presentation.