Practical Studies of Animal Development

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Product Details

  • ISBN-13: 9780470072417
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 1/1/1975
  • Edition number: 99
  • Pages: 251

Table of Contents

1 General Requirements.- Animals.- Legal considerations.- The laboratory.- Equipment.- Glassware.- Instruments.- Sources of information.- References.- 2 Echinoderms and Ascidians.- Echinoderms.- Induced spawning and artificial fertilization.- Ascidians.- Ciona intestinalis.- Dendrodoa grossularia.- References.- 3 Molluscs, Annelids and Nematodes.- Molluscs.- Patella.- Crepidula.- Littorina saxatilis.- Annelids.- Tubifex.- Pomaeros.- Nematodes.- Female reproductive system of Ascaris.- Development of Rhabditis.- References.- 4 Insects and Crustacea.- The Locust.- Life history.- Examination of the ovaries.- Development.- Calliphora.- Maintenance and life cycle.- Demonstration of hormonal control of metamorphosis.- Drosophila.- Life cycle and culture of larvae.- Observations of puffing patterns in the polytene chromosomes.- Crustacea.- Larval forms.- Culture of Artemia saliva.- References.- 5 Fish.- Goldfish.- Examination of the gonads.- Killifish.- General care.- Egg collection and rearing.- Development of Aphyosemion scheeli.- Guppies.- Artificial fertilization.- Experimental Work on Fish Embryos.- Decapsulation.- Explants of the blastoderm.- Experimental analysis of development.- References.- 6 Amphibia.- Xenopus.- Induced spawning.- Development.- Notes on feeding tadpoles.- Axolotls.- Spawning.- Development.- Notes on rearing from the larval stage.- Experiments on Amphibian Embryos.- Artificial fertilization.- Mechanical decapsulation.- Chemical decapsulation.- Exogastrulation.- Ectodermal explants from gastrulae.- Implantation of the dorsal lip of the blastopore.- Optic vesicle transplantation.- Other experiments on amphibian embryos.- Experiments on amphibian larvae.- Lens regeneration from the cornea.- Immunoelectrophoretic analysis of lens proteins.- Thyroxine induced regression of isolated tadpole tails.- References.- 7 Birds.- Examination of the early chick blastoderm.- Isolation of the blastoderm.- Stained preparations.- Culture of chick blastoderms.- Removal and culture of the blastoderm.- Preparation of chorio-allantoic grafts.- Isolation of limb buds.- Preparation of host embryos.- References.- 8 Mammals.- The oestrous cycle in the mouse.- Preparation of vaginal smears.- Examination of unfertilized ova from superovulated mice.- Superovulation.- Female reproductive system.- Isolation of living, unfertilized eggs.- Reproductive system of the male mouse.- Male reproductive system.- Examination of spermatozoa.- Examination of mouse embryos up to and including the blasyst stage.- Timing of matings.- Removal of eggs from the oviduct.- Removal of blasycts from the uterus.- In vitro culture of mouse eggs and egg fusion technique.- Culturing eight cell and earlier stage eggs.- Removal of the zona pellucida and fusion of eggs.- Arrangement of the foetal membranes in the rabbit and localization of immunoglobulin in the yolk sac splanchnopleur.- Examination of the rabbit conceptus.- Preparation of tissue for treatment with fluorescent labelled antibodies.- Immunofluorescent staining.- References.

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