Principles and Techniques of Biochemistry and Molecular Biology / Edition 6

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Overview

"This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained"--Provided by publisher.

"This textbook integrates the theoretical principles and experimental techniques common to all undergraduate courses in the bio- and medical sciences"--Provided by publisher.

Disc. general principles; specific techniques for cloning, protein structure, biomolecular interactions, more.

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Editorial Reviews

From the Publisher
"It is necessary to have a practical, up to date guide to experimental techniques at hand in a laboratory. This book belongs to such kind of texts. It covers all branches of basic as well as advanced techniques. The book represents a fundamental guidebook for work in a laboratory." --Photosynthetica
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Product Details

  • ISBN-13: 9780521828895
  • Publisher: Cambridge University Press
  • Publication date: 3/21/2005
  • Edition description: REV
  • Edition number: 6
  • Pages: 802
  • Product dimensions: 6.85 (w) x 9.72 (h) x 1.57 (d)

Meet the Author

Keith Wilson is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire.

John Walker is Professor and Head of the School of Life Sciences and also Head of the Science Training Centre at the University of Hertfordshire.

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Table of Contents

Preface; List of contributors; List of abbreviations; 1. Basic priniciples Keith Wilson and John Fyffe; 2. Cell culture techniques Anwar R. Baydoun; 3. Centrifugation Kay Ohlendieck; 4. Microscopy Stephen W. Paddock; 5. Molecular biology, bioinformatics and basic techniques Ralph Rapley; 6. Recombinant DNA and genetic analysis Ralph Rapley; 7. Immunochemical techniques Robin Thorpe and Susan Thorpe; 8. Protein structure, purification, characterisation and functional analysis John Walker; 9. Mass spectrometric techniques Alistair Aitken; 10. Electrophoretic techniques John Walker; 11. Chromatographic techniques Keith Wilson; 12. Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon; 13. Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields; 14. Radioisotope techniques Robert J. Slater; 15. Enzymes Keith Wilson; 16. Cell membrane receptors and cell signalling Keith Wilson.
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