Probing the Cytoplasm of Escherichia coli with Time-Resolved Fluorescence Anisotropy.

More About This Textbook


The fluorescence anisotropy of GFP was used to probe of the effect of high cellular biopolymer concentrations on rotational diffusion in E. coli. GFPmut2 expressed in the cytosol of E. coli K12 (MG1655) was studied using time-correlated single photon counting (TCSPC). The anisotropy of cytoplasmic GFP was found to decay as a single exponential with rotational correlation time of ϕ = 35 +/- 4 ns. The reduction factor for rotational diffusion of cytoplasmic GFP over GFP in buffer is Hr = D0/D rot ≈ 3. This is less than the reduction in translational diffusion (Ht ≈ 7) previously found for cytosolic GFPmut2 under similar cellular conditions. The rotational correlation time for GFP in solutions of increasing viscosity and solutions of increasing protein concentration were measured in order to quantify possible contributions of cellular viscosity and protein-protein interactions to Hr. The reduction in rotational diffusion could not be explained by either of these effects alone. We also describe the TCSPC microscope built to carry out these measurements, its use in collected TCSPC data, and a description of the software created for analysis of the TCSPC data.
Read More Show Less

Product Details

  • BN ID: 2940043615879
  • Publisher: ProQuest LLC
  • Sold by: Barnes & Noble
  • Format: eTextbook
  • Pages: 97

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)