Prokaryotic Genomics

by Michel Blot (Editor)

Overview

Prokaryotic Genomics provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and new tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology.

Product Details

ISBN-13:	9783764365967
Publisher:	Birkhäuser Basel
Publication date:	02/12/2003
Series:	Methods and Tools in Biosciences and Medicine
Edition description:	Softcover reprint of the original 1st ed. 2003
Pages:	208
Product dimensions:	7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

1 Physical Analysis of Chromosome Size Variation.- 2 Genetic Mapping in Salmonella enterica.- 3 Insertion Sequences as Genomic Markers.- 4 The Use of Noncoding Microsatellite Length Analysis for Bacterial Strain Typing.- 5 How to Amplify Easily, on the Bacterial Chromosome, a Desired DNA Sequence.- 6 Generalized Transduction.- 7 Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions.- 8 Genetic Footprinting for Bacterial Functional Genomics.- 9 Gene Transfer to Plants through Bacterial Vectors.- 10 Quorum Sensing: Approaches to Identify Signals and Signalling Genes in Gram-negative Bacteria.- 11 Transcriptional Profiling in Bacteria Using Microarrays.- 12 Transcriptome Analysis by Macroarrays.- 13 Prokaryotic Proteomics.- 14 Intein-mediated Protein Purification.- 15 Two-hybrid Assay in Escherichia coli K12.- Guide to Prools.