Protein Function: A Practical Approach / Edition 2

Protein Function: A Practical Approach / Edition 2

by T. E. Creighton
     
 

ISBN-10: 019963615X

ISBN-13: 9780199636150

Pub. Date: 04/28/1997

Publisher: Oxford University Press, USA

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice

Overview

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

Product Details

ISBN-13:
9780199636150
Publisher:
Oxford University Press, USA
Publication date:
04/28/1997
Series:
Practical Approach Series, #175
Edition description:
REV
Pages:
360
Product dimensions:
6.19(w) x 9.25(h) x 0.77(d)

Table of Contents

1. Identifying proteins for proteome studies: a two-dimensional gel electrophoresis approach
2. Expressing cloned genes in Escherichia coli
3. Folding proteins
4. Ligand binding
5. Ligand blotting
6. Affinity labelling
7. Chemical cross-linking and protein function
8. Analysis of sequence-specific DNA-binding proteins
9. Purification of sequence-specific DNA-binding proteins by affinity chromatography
10. Chemical modification

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