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Protein Function: A Practical Approach / Edition 2

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Overview

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

The book contains black-and-white illustrations.

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Product Details

  • ISBN-13: 9780199636150
  • Publisher: Oxford University Press, USA
  • Publication date: 4/28/1997
  • Series: Practical Approach Series , #175
  • Edition description: REV
  • Edition number: 2
  • Pages: 360
  • Product dimensions: 6.19 (w) x 9.25 (h) x 0.77 (d)

Table of Contents

List of Contributors
Abbreviations
1 Identifying proteins for proteome studies: a two-dimensional gel electrophoresis approach 1
2 Expressing cloned genes in Escherichia coli 29
3 Folding proteins 57
4 Ligand binding 101
5 Ligand blotting 131
6 Affinity labelling 155
7 Chemical cross-linking and protein function 185
8 Analysis of sequence-specific DNA-binding proteins 215
9 Purification of sequence-specific DNA-binding proteins by affinity chromatography 245
10 Chemical modification 279
Appendix 317
Index 323
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