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The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.
The book contains black-and-white illustrations.
|List of Contributors|
|1||Identifying proteins for proteome studies: a two-dimensional gel electrophoresis approach||1|
|2||Expressing cloned genes in Escherichia coli||29|
|7||Chemical cross-linking and protein function||185|
|8||Analysis of sequence-specific DNA-binding proteins||215|
|9||Purification of sequence-specific DNA-binding proteins by affinity chromatography||245|