Protein Nanotechnology: Protocols, Instrumentation, and Applications / Edition 1

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Leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens.

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Product Details

  • ISBN-13: 9781588293107
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 1/20/2005
  • Series: Methods in Molecular Biology Series , #300
  • Edition description: 2005
  • Edition number: 1
  • Pages: 466
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.06 (d)

Table of Contents

Preface v
Acknowledgments vii
Contributors xi
1 Protein Nanotechnology: The New Frontier in Biosciences 1
2 Kinetics and Mechanisms of Protein Crystallization at the Molecular Level 15
3 Nanostructured Systems for Biological Materials 53
4 Nanomaterials of Drug Delivery Systems for Tissue Regeneration 81
5 Nanotechnology With S-Layer Proteins 101
6 Folding of [beta]-Structured Fibrous Proteins and Self-Assembling Peptides 125
7 Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors 141
8 Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy 165
9 Carbon Nanotubes and Nanowires for Biological Sensing 191
10 Carbon Nanotube Systems to Communicate With Enzymes 225
11 Molecularly Imprinted Polymers for Biomolecular Recognition 243
12 Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis 255
13 Bacterial Virus [phi]29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology 285
14 Construction of Ordered Protein Arrays 325
15 Bioengineering and Characterization of DNA-Protein Assemblies Floating on Supported Membranes 349
16 Nanosystems for Biosensing: Multianalyte Immunoassay on a Protein Chip 369
17 Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells 383
18 Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity 403
19 Protein Amyloidose Misfolding: Mechanisms, Detection, and Pathological Implications 417
20 Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution 437
Index 453
About the Author 465
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