Protein Purification Protocols / Edition 2

by Paul Cutler

Overview

The first edition of Protein Purification Prools (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of prools that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Product Details

ISBN-13:	9781588290670
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/12/2003
Series:	Methods in Molecular Biology , #244
Edition description:	2nd ed. 2004
Pages:	484
Product dimensions:	7.01(w) x 10.00(h) x 0.05(d)

Table of Contents

General Strategies.- Preparation of Extracts From Animal Tissues.- Protein Extraction From Plant Tissues.- Extraction of Recombinant Protein From Bacteria.- Protein Extraction From Fungi.- Subcellular Fractionation of Animal Tissues.- Subcellular Fractionation of Plant Tissues.- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds.- Avoidance of Proteolysis in Extracts.- Concentration of Extracts.- Making and Changing Buffers.- Purification and Concentration by Ultrafiltration.- Bulk Purification by Fractional precipitation.- Ion-Exchange Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- Lectin Affinity Chromatography.- Immunoaffinity Chromatography.- Immobilized Metal-Ion Affinity Chromatography.- Chromatography on Hydroxyapatite.- Thiophilic Affinity Chromatography and Related Methods.- Affinity Precipitation Methods.- Isoelectric Focusing.- Chromatofocusing.- Size-Exclusion Chromatography.- Fast Protein Liquid Chromatography.- Reversed-Phase Chromatography of Proteins.- Extraction of Membrane Proteins.- Removal of Detergent From Protein Fractions.- Purification of Membrane Proteins.- Lyophilization of Proteins.- Storage of Pure Proteins.- Electroelution of Proteins From Polyacrylamide Gels.- Electroblotting of Proteins From Polyacrylamide Gels.- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses.- Microscale Solution Isoelectrofocusing.- Practical Column Chromatography.- Detection Methods.- Peptide Proteomics.- Multidimensional Liquid Chromatography of Proteins.- Mass Spectrometry.- Purification of Therapeutic Proteins.- Purification Process Scale-Up.

Editorial Reviews

From reviews of the first edition...

"...very useful..."

-Angewandte Chemie: International Edition

"...valuable to keep around the laboratory..."

-Biopharm

"...recommended for protein chemists....There is something in this book for

everyone....an excellent working book as well as an excellent reference."

-JAOCS: Journal of the American Oil Chemists' Society

"...an excellent lab companion..."

-SIM News

"...a worthwhile purchase for novice and established practitioners alike."

-Microbiology Today

"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."

-Biomedical Chromatography

"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified prools. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of prools useful for both less and more experienced practitioners."-SGM Quarterly

From the Publisher