Protein Purification: Principles and Practice / Edition 3

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The third edition of this classic guide to protein purification update s methods, principles, and references. As in the widely acclaimed earl ier editions, Scopes guides both the novice and the experienced resear cher from theory to application. Using the book, the reader will be ab le to integrate methods effectively into optimum protocols for the tas k at hand.

The third edition of this classic guide updates methods, principles, and references, taking both the novice and the experience researcher from theory to application. "Should be on every laboratory shelf where proteins are being handled or purified . . . a feast and a genuine pleasure to read."--Nature. 164 illustrations.

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Editorial Reviews

A guide providing a brief account of the main protein fractionation methods, with some simple theoretical and thermodynamic explanations of the events occurring, for students and researchers involved in the process of isolating an enzyme, from whatever source. A basic background in biochemistry and protein chemistry is assumed. This updated edition (2nd ed., 1987) adds new material on purification of special protein types (membrane proteins, recombinant proteins, antibodies) and on affinity chromatography. Annotation c. Book News, Inc., Portland, OR (
From the Publisher
From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)
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Product Details

  • ISBN-13: 9780387940724
  • Publisher: Springer New York
  • Publication date: 11/19/1993
  • Series: Springer Advanced Texts in Chemistry Series
  • Edition description: 3rd ed. 1994
  • Edition number: 3
  • Pages: 400
  • Sales rank: 538,793
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.00 (d)

Table of Contents

Prefaces; 1. The Protein Purification Laboratory; 2. Making an Extract; 3. Analysis- Measurement of Protein and Enzyme Activity; 4. Separation by Precipitation; 5. Separation by Adsorption I: General Principles; 6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents; 7. Separation by Adsorption: Affinity Techniques; 8. Separation in Solution; 9. Purification of Special Types of Proteins; 10. Small-Scale and Large-Scale Procedures; 11. Analysis for Purity; 12. Optimization of Procedures: Final Steps; Appendix A-D; References; Index

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