Protein Sequencing and Identification Using Tandem Mass Spectrometry / Edition 1

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Overview

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry
The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.
Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:
* Peptide fragmentation and interpretation of product ion spectra
* Basic polyacrylamide gel electrophoresis
* Preparation of protein digests for sequencing experiments
* Mass spectrometric analysis using capillary liquid chromatography
* Techniques for protein identification by database searches
* Characterization of modified peptides using tandem mass spectrometry
And much more

Author Biography: MICHAEL KINTER, PhD, is an associate staff member in the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation.
NICHOLAS E. SHERMAN, PhD, is anassistant professor of research in the W. M. Keck Biomedical Mass Spectrometry Laboratory, Department of Microbiology, University of Virginia.

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Editorial Reviews

SciTech Book
[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods...
SciTech Book
[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods...
Booknews
Kinter (Cleveland Clinic Foundation) and Sherman (U. of Virginia) present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods along with information about the proper selection and operation of instruments and the fundamental principles of mass spectrometry and protein sequencing. Among the topics: peptide fragmentation and interpretation of product ion spectra, basic polycrylamide gel electrophoresis, preparation of protein digests for sequencing experiments, mass spectrometric analysis using capillary liquid chromatography, techniques for protein identification by database searches, and characterization of modified peptides using tandem mass spectrometry. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher
"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001)

"...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)

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Product Details

  • ISBN-13: 9780471322498
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 10/2/2000
  • Series: Wiley Series on Mass Spectrometry Series , #2
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 320
  • Sales rank: 1,329,928
  • Product dimensions: 6.36 (w) x 9.53 (h) x 0.78 (d)

Meet the Author

MICHAEL KINTER, PhD, is an associate staff member in the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation.

NICHOLAS E. SHERMAN, PhD, is an assistant professor of research in the W. M. Keck Biomedical Mass Spectrometry Laboratory, Department of Microbiology, University of Virginia.

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Table of Contents

Series Preface
Preface
Ch. 1 An Introduction to Protein Sequencing Using Tandem Mass Spectrometry 1
Ch. 2 The Primary Structure of Proteins and a Historical Overview of Protein Sequencing 6
Ch. 3 Fundamental Mass Spectrometry 29
Ch. 4 Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra 64
Ch. 5 Basic Polyacrylamide Gel Electrophoresis 117
Ch. 6 The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments 147
Ch. 7 Mass Spectrometric Analysis of Tryptic Digests 166
Ch. 8 Protein Identification by Database Searching 207
Ch. 9 Sequence Analysis of Novel Proteins 238
Ch. 10 The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry 269
Index 295
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