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Author Biography: MICHAEL KINTER, PhD, is an associate staff member in the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation.
NICHOLAS E. SHERMAN, PhD, is anassistant professor of research in the W. M. Keck Biomedical Mass Spectrometry Laboratory, Department of Microbiology, University of Virginia.
"...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)
|Ch. 1||An Introduction to Protein Sequencing Using Tandem Mass Spectrometry||1|
|Ch. 2||The Primary Structure of Proteins and a Historical Overview of Protein Sequencing||6|
|Ch. 3||Fundamental Mass Spectrometry||29|
|Ch. 4||Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra||64|
|Ch. 5||Basic Polyacrylamide Gel Electrophoresis||117|
|Ch. 6||The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments||147|
|Ch. 7||Mass Spectrometric Analysis of Tryptic Digests||166|
|Ch. 8||Protein Identification by Database Searching||207|
|Ch. 9||Sequence Analysis of Novel Proteins||238|
|Ch. 10||The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry||269|