Protein Sequencing Protocols

Overview

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, Protein Sequencing Prools, Second Edition, provides for both novice and ...

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Overview

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, Protein Sequencing Prools, Second Edition, provides for both novice and expert investigators alike a ready source of easy-to-follow prools that simplify choosing the most appropriate method for protein sequence determination.

The book contains black-and-white illustrations.

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Editorial Reviews

From the Publisher
"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics

"A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research

From Reviews of the First Edition...

"...full of valuable information..." -Biochemical Education

"...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters

"...an extremely useful compendium of protein analysis prools..." -Nahrung-Food

"...well organized and accessible..." -Journal of the American Chemical Society

"The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology

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Product Details

  • ISBN-13: 9781617372841
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Biology Series , #211
  • Edition description: Softcover reprint of hardcover 2nd ed. 2003
  • Edition number: 2
  • Pages: 493
  • Product dimensions: 6.00 (w) x 9.00 (h) x 1.02 (d)

Table of Contents

Strategies for Handling Polypeptides on a Micro-Scale
Bryan John Smith and Paul Tempst

SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing
Bryan John Smith

Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization
Michael J. Dunn

Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization
Michael J. Dunn

Enzymatic Cleavage of Proteins
Bryan John Smith

Chemical Cleavage of Polypeptides
Bryan John Smith

Enzymatic Preparation and Isolation of Glycopeptides
David J. Harvey

Analytical and Micropreparative Capillary Electrophoresis of Peptides
Alan J. Smith

High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues
Christoph W. Turck

Hydrolysis of Samples for Amino Acid Analysis
Ian Davidson

Amino Acid Analysis: Precolumn Derivatization Methods
G. Brent Irvine and Ian Davidson

Post Column Amino Acid Analysis
Alan J. Smith

Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples
Steven A. Cohen

Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
Petr Jandik, Jun Cheng, and Nebojsa Avdalovic

d-Amino Acid Analysis
Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa

Validation of Amino Acid Analysis Methods
Andrew J. Reason

Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins
Fiona M. Greer and Howard R. Morris

Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
Darryl J. C. Pappin

Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes
Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, and Andrej Shevchenko

Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis
David M. Schieltz and John R. Yates, III

Identification of PTH-Amino Acids by HPLC
Gregory A. Grant and Mark W. Crankshaw

Protein Sequencer Maintenance and Troubleshooting
Bryan Dunbar

N-Terminal Protein Sequencing for Special Applications
Philip J. Jackson

Identification of Phosphorylation Sites by Edman Degradation
Laurey Steinke and Richard G. Cook

Validation of Protein Sequencing in a Regulated Laboratory
Philip J. Jackson and Neil Dodsworth

Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method
David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham

Chemical Modifications of Proteins as an Aid to Sequence Analysis
Alex F. Carne

Deblocking of N-Terminally Modified Proteins
Hisashi Hirano and Roza M. Kamp

Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid
Jacek Mozdzanowski

Identification of Sites of Protein Glycosylation
David J. Harvey

Analysis of Sites of Protein Phosphorylation
Alastair Aitken and Michele Learmonth

Quantitation and Location of Disulfide Bonds in Proteins
Alastair Aitken and Michele Learmonth

Getting the Most from Your Protein Sequence
Richard R. Copley and Robert B. Russell

Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids
James P. Turner

Appendix 2: Protein Consensus Sequence Motifs
Alastair Aitken

Index

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