Protein Synthesis: Methods and Protocols / Edition 1

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Overview

This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Juergen Mollenhauer, PhD, DSc (Rush Medical College of Rush University)
Description: This book focuses on the major events around translation: ribosomal structure-function relationships; tRNA; rRNA; errors in protein synthesis; modified tRNAs; modified amino acids; dynamics of translation; in vitro translation; and drug utilization. In short, this is a description of the protein synthesis machinery.
Purpose: The editor presents the book as "a collection of core methods and protocols .... to illuminate the inner workings of the protein synthetic machinery." These are interesting and important objectives, considering the naivete of molecular biologists expressing their proteins of interest here, there, and everywhere. The book is carefully edited, the chapters are well integrated, and the book delivers on the editor's promise.
Audience: The targeted readers are highly experienced experimenters. Although the book can be read by many others, the emphasis on methods leaves little room for general explanations of the subject of research. Only those with an intimate knowledge of the basics of protein synthesis will fully profit.
Features: The title is somewhat misleading. Exploration of protein synthesis is not really the issue, rather the tools of the synthesis machinery are covered. Perhaps a second edition should be renamed. On the other hand, a well-equipped biochemical laboratory might decide to test one of the approaches presented here to solve problems rather than something less sophisticated. There is little missing, except perhaps an extended, cross-referenced index that would include company names providing the materials used (though the contributors do cite these companies in the context of the material sections).
Assessment: The overall quality is very high. Even compared to similar approaches (such as the volumes of the Methods in Enzymology series), this book is still outstanding in its details.
Juergen Mollenhauer
This book focuses on the major events around translation: ribosomal structure-function relationships; tRNA; rRNA; errors in protein synthesis; modified tRNAs; modified amino acids; dynamics of translation; in vitro translation; and drug utilization. In short, this is a description of the protein synthesis machinery. The editor presents the book as "a collection of core methods and protocols .... to illuminate the inner workings of the protein synthetic machinery." These are interesting and important objectives, considering the naivete of molecular biologists expressing their proteins of interest here, there, and everywhere. The book is carefully edited, the chapters are well integrated, and the book delivers on the editor's promise. The targeted readers are highly experienced experimenters. Although the book can be read by many others, the emphasis on methods leaves little room for general explanations of the subject of research. Only those with an intimate knowledge of the basics of protein synthesis will fully profit. The title is somewhat misleading. Exploration of protein synthesis is not really the issue, rather the tools of the synthesis machinery are covered. Perhaps a second edition should be renamed. On the other hand, a well-equipped biochemical laboratory might decide to test one of the approaches presented here to solve problems rather than something less sophisticated. There is little missing, except perhaps an extended, cross-referenced index that would include company names providing the materials used (though the contributors do cite these companies in the context of the material sections). The overall quality is very high. Even compared to similarapproaches (such as the volumes of the Methods in Enzymology series), this book is still outstanding in its details.
Booknews
A collection of molecular biology methods specific to protein synthesis introduced by a summary of basic background information, strategy, and methodological details. Highlights include the analysis of the fidelity and movement of ribosomes, protocols for preparing protein synthesis, methods for incorporating non-standard amino acids, and cRNA structure at the nucleotide level. Bacterial, fungal, plant, and animal systems are considered. Biochemists and molecular and cell biologists are the expected readership. Annotation c. by Book News, Inc., Portland, Or.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780896033979
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/4/1998
  • Series: Methods in Molecular Biology Series , #77
  • Edition description: 1998
  • Edition number: 1
  • Pages: 442
  • Product dimensions: 9.00 (w) x 6.00 (h) x 1.19 (d)

Table of Contents

Analyses of Ribosome Distribution During In Vitro Translation, Sandra L. Wolin. Allele-Specific Priming in the Mapping of rRNA, Stephen Douthwaite. Permeabilized Mammalian Cells as a System for Protein Synthesis,

Romualdas Stapulionis and Murray P. Deutscher. The Measurement of Processivity Errors in Protein Synthesis,

Hengjiang Dong. A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins,

Jon S. Thorson, Virginia W. Cornish, Jeannie E. Barrett,

Sharon T. Cload, Takato Yano, and Peter G. Schultz. The Analysis of Translational Activity Using a Reporter Gene

Constructed from Repeats of an Antibody-Binding Domain

from Protein A, Asgeir Björnsson, Salim Mottagui-Tabar, and Leif A. Isaksson. In Vitro Engineering Using Acyl-Derivatized tRNAs, Obed W. Odom, Wieslaw Kudlicki, and Boyd Hardesty. In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anodons, Gisela Kramer, Wieslaw Kudlicki, and Boyd Hardesty. Analysis of rRNA Function Using Specialized Ribosomes, Marcel F. Brink and Herman A. de Boer. Determination of the Peptide Elongation Rate In Vivo, Michael A. Sørensen and Steen Pedersen. The Measurement of Missense Errors During In Vivo Protein Synthesis, Bernard N. Violand and Gregg Bogosian. Heelprinting Analysis of In Vivo Ribosome Pause Sites,

Margaret J. Hollingsworth, Jeong-Kook Kim,

and Neil E. Stollar. Analysis of eIF-2a Kinases in Yeast,

Thomas E. Dever. Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems, Lyubov A. Ryabova, Igor Yu. Morozov,

and Alexander S. Spirin. Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis, Virginia M. Pain, Tina D. Patrick, Richard Cox,

and Simon J. Morley. A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors,

Michael Rau, Theophile Ohlmann, Virginia M. Pain,

and Simon J. Morley. Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems, Måns Ehrenberg and Ne¸'e Bilgin. Measurement of Rate of Protein Synthesis In Vitro Preparation of Escherichia coli Burst Systems, Ne¸'e Bilgin and Måns Ehrenberg. In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae, Jaap Venema, Rudi J. Planta, and Hendrik A. Raué. Genetic Selection of rRNA Mutations, Steven T. Gregory, Carleen A. Brunelli, J. Stephen Lodmell, Michael O'Connor, and Albert E. Dahlberg. Toeprinting Assays: Mapping by Blocks to Reverse Transcriptase Primer Extension, Steven Ringquist and Larry Gold. Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution,

Andrey V. Bakin and James Ofengand. Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes,

Cristina Rodriguez-Fonseca and Roger A. Garrett. Protein Engineering with Nonstandard Amino Acids. Lance E. Steward and A. Richard Chamberlin. Internal Ribosome Entry Sites Tests with Circular mRNAs, Chang-you Chen and Peter Sarnow. Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5' or 3' UTRs of Translationally Regulated mRNAs, James Walker, Osvaldo de Melo Neto, and Nancy Standart. Translational Control by Repressor Proteins Binding

to the 5' UTR of mRNAs, Nicola K. Gray. Identification and Analysis of Frameshift Sites, Arunachalam Vimaladithan and Philip J. Farabaugh. Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions, Marylena Dabrowski and Knud H. Nierhaus.

Index.

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