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This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the ...
This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.
The book contains black-and-white illustrations.
Analyses of Ribosome Distribution During In Vitro Translation, Sandra L. Wolin. Allele-Specific Priming in the Mapping of rRNA, Stephen Douthwaite. Permeabilized Mammalian Cells as a System for Protein Synthesis,
Romualdas Stapulionis and Murray P. Deutscher. The Measurement of Processivity Errors in Protein Synthesis,
Hengjiang Dong. A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins,
Jon S. Thorson, Virginia W. Cornish, Jeannie E. Barrett,
Sharon T. Cload, Takato Yano, and Peter G. Schultz. The Analysis of Translational Activity Using a Reporter Gene
Constructed from Repeats of an Antibody-Binding Domain from Protein A, Asgeir Björnsson, Salim Mottagui-Tabar, and Leif A. Isaksson. In Vitro Engineering Using Acyl-Derivatized tRNAs, Obed W. Odom, Wieslaw Kudlicki, and Boyd Hardesty. In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anodons, Gisela Kramer, Wieslaw Kudlicki, and Boyd Hardesty. Analysis of rRNA Function Using Specialized Ribosomes, Marcel F. Brink and Herman A. de Boer. Determination of the Peptide Elongation Rate In Vivo, Michael A. Sørensen and Steen Pedersen. The Measurement of Missense Errors During In Vivo Protein Synthesis, Bernard N. Violand and Gregg Bogosian. Heelprinting Analysis of In Vivo Ribosome Pause Sites,
Margaret J. Hollingsworth, Jeong-Kook Kim,
and Neil E. Stollar. Analysis of eIF-2a Kinases in Yeast,
Thomas E. Dever. Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems, Lyubov A. Ryabova, Igor Yu. Morozov,
and Alexander S. Spirin. Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis, Virginia M. Pain, Tina D. Patrick, Richard Cox,
and Simon J. Morley. A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors,
Michael Rau, Theophile Ohlmann,Virginia M. Pain,
and Simon J. Morley. Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems, Måns Ehrenberg and Ne¸'e Bilgin. Measurement of Rate of Protein Synthesis In Vitro Preparation of Escherichia coli Burst Systems, Ne¸'e Bilgin and Måns Ehrenberg. In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae, Jaap Venema, Rudi J. Planta, and Hendrik A. Raué. Genetic Selection of rRNA Mutations, Steven T. Gregory, Carleen A. Brunelli, J. Stephen Lodmell, Michael O'Connor, and Albert E. Dahlberg. Toeprinting Assays: Mapping by Blocks to Reverse Transcriptase Primer Extension, Steven Ringquist and Larry Gold. Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution,
Andrey V. Bakin and James Ofengand. Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes,
Cristina Rodriguez-Fonseca and Roger A. Garrett. Protein Engineering with Nonstandard Amino Acids. Lance E. Steward and A. Richard Chamberlin. Internal Ribosome Entry Sites Tests with Circular mRNAs, Chang-you Chen and Peter Sarnow. Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5' or 3' UTRs of Translationally Regulated mRNAs, James Walker, Osvaldo de Melo Neto, and Nancy Standart. Translational Control by Repressor Proteins Binding to the 5' UTR of mRNAs, Nicola K. Gray. Identification and Analysis of Frameshift Sites, Arunachalam Vimaladithan and Philip J. Farabaugh. Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions, Marylena Dabrowski and Knud H. Nierhaus.