Proteome Research: Mass Spectrometry / Edition 1

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Recent advances in large scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. This is the first book to extensively cover the applications of mass spectrometry to proteome research.

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Product Details

  • ISBN-13: 9783540672562
  • Publisher: Springer Berlin Heidelberg
  • Publication date: 12/12/2000
  • Series: Principles and Practice Series
  • Edition description: Softcover reprint of the original 1st ed. 2001
  • Edition number: 1
  • Pages: 274
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.63 (d)

Table of Contents

1 Mass Spectrometry and the Proteome
2 Basics of Triple-Stage Quadrupole/Ion-Trap Mass Spectrometry: Precursor, Product and Neutral-Loss Scanning. Electrospray Ionisation and Nanospray Ionisation
3 The Basics of Matrix-Assisted Laser Desorption, Ionisation Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis
4 Data-Controlled Micro-Scale Liquid Chromatography - Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness
5 Solid-Phase Extraction - Capillary Zone Electrophoresis-Mass Spectrometry Analysis of Low-Abundance Proteins
6 Protein Identification by Peptide-Mass Fingerprinting
7 Protein Identification by SEQUEST
8 Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra
9 Automated Interpretation of Peptide Tandem Mass Spectra and Homology Searching
10 Specific Detection and Analysis of Phosphorylated Peptides by Mass Spectrometry
11 Glycoproteomics: High-Throughput Sequencing of Oligosaccharide Modifications to Proteins
12 Proteomics Databases
13 Quo Vadis
Subject Index
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