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Protocols for Adult Stem Cells / Edition 1
     

Protocols for Adult Stem Cells / Edition 1

by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li
 

ISBN-10: 1607610620

ISBN-13: 9781607610625

Pub. Date: 01/26/2010

Publisher: Springer-Verlag New York, LLC

The study of adult stem cells has surged in recent years. Because they are responsible for the body's natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult

Overview

The study of adult stem cells has surged in recent years. Because they are responsible for the body's natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular BiologyT series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Product Details

ISBN-13:
9781607610625
Publisher:
Springer-Verlag New York, LLC
Publication date:
01/26/2010
Series:
Methods in Molecular Biology Series , #621
Edition description:
2010
Pages:
207
Product dimensions:
7.20(w) x 10.10(h) x 0.80(d)

Table of Contents

Preface v

Contributors ix

1 In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells Rodrigo Fernandez-Gonzalez Irineu Illa-Bochaca Dawne N. Shelton Bryan E. Welm Mary Helen Barcellos-Hoff Carlos Ortiz-de-Solorzano 1

2 Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies Irineu Illa-Bochaca Rodrigo Fernandez-Gonzalez Dawne N. Shelton Bryan E. Welm Carlos Ortiz-de-Solorzano Mary Helen Barcellos-Hoff 29

3 Use of Stem Cell Markers in Dissociated Mammary Populations Dawne N. Shelton Rodrigo Fernandez-Gonzalez Irineu Illa-Bochaca Carlos Ortiz-de-Solorzano Mary Helen Barcellos-Hoff Bryan E. Welm 49

4 Isolation of Adult Hippocampal Neural Progenitors Joseph Peltier Brandi K. Ormerod David V. Schaffer 57

5 In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors Joseph Peltier Smita Agrawal Matthew J. Robertson David V. Schaffer 65

6 In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors Matthew J. Robertson Joseph Peltier David V. Schaffer 89

7 Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors Joseph Peltier David V. Schaffer 103

8 Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix Jennifer S. Park Craig Hashi Song Li 117

9 Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis Steve Khachi Yiqian Zhu Guo-Yuan Yang 139

10 Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells Michael J. Conboy Irina M. Conboy 149

11 Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells Michael J. Conboy Massimiliano Cerletti Amy J. Wagers Irina M. Conboy 165

12 Altered Expression of NFκB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy Anshu Agrawal 175

13 Preparation of Collagen-Coated Gels that Maximize in Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of in Vivo Muscle Tathagata Chaudhuri Florian Rehfeldt H. Lee Sweeney Dennis E. Discher 185

Index 203

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