Protocols For Neural Cell Culture / Edition 2

Paperback (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $36.65
Usually ships in 1-2 business days
(Save 63%)
Other sellers (Paperback)
  • All (2) from $36.65   
  • Used (2) from $36.65   
Close
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any BN.com coupons and promotions
$36.65
Seller since 2014

Feedback rating:

(69)

Condition:

New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.

Good
1996 Trade paperback 2nd ed. Good. The book has been read but remains in clean condition. All pages are intact and the cover is intact. Some minor wear to the spine. Trade ... paperback (US). Glued binding. 280 p. *****PLEASE NOTE: This item is shipping from an authorized seller in Europe. In the event that a return is necessary, you will be able to return your item within the US. To learn more about our European sellers and policies see the BookQuest FAQ section***** Read more Show Less

Ships from: Goring-By-Sea, United Kingdom

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
$54.45
Seller since 2014

Feedback rating:

(266)

Condition: Good
Possible retired library copy, some have markings or writing. May or may not include accessories such as CD or access codes.

Ships from: Chatham, NJ

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
Page 1 of 1
Showing All
Close
Sort by

Overview

This second edition of a best-selling and highly praised collection of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection. This book's unique wealth of practical detail and its inclusion of a wide range of tissue culture systems having many applications ensure that it will remain "a valuable resource that will find its place on many laboratory shelves (Trends in Biotechnology)".

The book contains black-and-white illustrations.

Read More Show Less

Editorial Reviews

Doody's Review Service
Reviewer: Alexander Kolchinsky, PhD (University of Illinois at Chicago College of Medicine)
Description: The first 12 chapters in this manual cover slice cultures, explants, and primary cell cultures from animals that are widely used in neuroscience, as well as human biopsies. Eight additional chapters discuss various methods of analysis, from general tips on recommended equipment and routine techniques to sophisticated microscopy and gene therapy experiments using herpesvirus. The editors have achieved consistent format of chapters with very moderate overlapping.
Purpose: The purpose of the book is to provide a practical manual for experimentation in neuroscience. The new edition is updated by several new chapters. It may serve as a benchtop laboratory manual and reference. In addition, it offers a fresh look at hot topics of neuroscience. A good example is the a brief discussion of the presence of astroglial precursors in brain cultures (chapter 11).
Audience: The potential audience for the book ranges from a principal investigator of a project to a part-time undergraduate working in a neuroscience lab. Anyone interested in neuroscience can find descriptions of basic experimental facts and their immediate interpretation.
Features: The book is clearly written and gives all the necessary experimental details. It has a list of abbreviations and detailed subject index. Lists of references contain mostly appropriate books and reviews. Black-and-white photographs are of high quality and very informative. They include microphotographs and schemes and sometimes illustrate the organization of the workplace.
Assessment: Most chapters present an outline of the possible goals of specific experiments. The authors wisely refrain from detailed discussions of issues; instead, they lay strong laboratory background for a wide variety of studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience.
Alexander Kolchinsky
The first 12 chapters in this manual cover slice cultures, explants, and primary cell cultures from animals that are widely used in neuroscience, as well as human biopsies. Eight additional chapters discuss various methods of analysis, from general tips on recommended equipment and routine techniques to sophisticated microscopy and gene therapy experiments using herpesvirus. The editors have achieved consistent format of chapters with very moderate overlapping. The purpose of the book is to provide a practical manual for experimentation in neuroscience. The new edition is updated by several new chapters. It may serve as a benchtop laboratory manual and reference. In addition, it offers a fresh look at hot topics of neuroscience. A good example is the a brief discussion of the presence of astroglial precursors in brain cultures (chapter 11). The potential audience for the book ranges from a principal investigator of a project to a part-time undergraduate working in a neuroscience lab. Anyone interested in neuroscience can find descriptions of basic experimental facts and their immediate interpretation. The book is clearly written and gives all the necessary experimental details. It has a list of abbreviations and detailed subject index. Lists of references contain mostly appropriate books and reviews. Black-and-white photographs are of high quality and very informative. They include microphotographs and schemes and sometimes illustrate the organization of the workplace. Most chapters present an outline of the possible goals of specific experiments. The authors wisely refrain from detailed discussions of issues; instead, they lay strong laboratory background for a wide varietyof studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience.
Booknews
This updated manual featuring reproducible neural tissue culture protocols for established laboratory procedures adds 11 chapters describing important new techniques: e.g. the isolation, growth, and characterization of neural stem cells; the production and purification of monoclonal antibodies; hippocampal and microglial slice cultures; and transfection of neurons in culture with adenovirus. Chapter 27 concludes with tips for tissue culture. Detailed dissection procedures are a plus for scientists not trained in neuroanatomy. Prior editions appeared in 1992 and 1997. The editors are in the department of anatomy and cell biology at the U. of Saskatchewan's School of Medicine. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

4 Stars! from Doody
Read More Show Less

Product Details

  • ISBN-13: 9780896034549
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/1/1996
  • Edition description: Older Edition
  • Edition number: 2
  • Pages: 300
  • Product dimensions: 0.63 (w) x 11.00 (h) x 8.25 (d)

Table of Contents

Introduction, Marston Manthorpe. Outgrowth Assays and Cortical Slice Cultures, Valérie Castellani and Jürgen Bolz. Microexplant Cultures of the Cerebellum, Bernard Rogister and Gustave Moonen. Aggregating Neural Cell Cultures, Paul Honegger and Florianne Monnet-Tschudi. Primary Cell Cultures of Peripheral and Central Neurons and Glia, Mary I. Johnson and Richard P. Bunge. Chick Spinal Somatic Motoneurons in Culture, Bernhard H. J. Juurlink. Neural Cells in Microcultures: In Vitro Biological Assays for Neuroactive Agents, Marston Manthorpe. Construction and Use of Compartmented Cultures, Robert B. Campenot. Astrocyte and Oligodendrocyte Cultures, Ruth Cole and Jean de Vellis. Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium, Sergey Fedoroff and Arleen Richardson. Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium, Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon. Culture of Glial Cells from Human Brain Biopsies, Voon Wee Yong and Jack P. Antel. Colony Cultures, Sergey Fedoroff and Arleen Richardson. Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System , Angela P. Dyer and Frank Tufaro. Immunostaining and Identification of Antigens, Colin J. Barnstable. Elimination of Cell Types from Mixed Neural Cell Cultures, Richard M. Devon. Quantification of Cells in Culture, Arleen Richardson and Sergey Fedoroff. Procedures for Subculturing Cells, Arleen Richardson and Sergey Fedoroff. Preparation of Tissue-Cultured Material for Electron Microscopical Observation, Richard M. Devon. Tips in Tissue Culture, Sergey Fedoroff and Arleen Richardson. Use of Dissecting Instruments in Tissue Culture, Arleen Richardson. Abbreviations and Definitions. Index.
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)