Protoplast Fusion: Genetic Engineering in Higher Plants

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Product Details

  • ISBN-13: 9783642822476
  • Publisher: Springer Berlin Heidelberg
  • Publication date: 12/27/2011
  • Series: Monographs on Theoretical and Applied Genetics Series, #8
  • Edition description: Softcover reprint of the original 1st ed. 1984
  • Edition number: 1
  • Pages: 222
  • Product dimensions: 6.69 (w) x 9.61 (h) x 0.50 (d)

Table of Contents

1 Introduction.- 2 Techniques of Parasexual Hybridization.- 2.1 Culture Techniques.- 2.1.1 Culture of Isolated Plant Protoplasts.- 2.1.2 Culture of Single Cells and Protoplasts.- 2.2 Isolated Protoplast Stage and Plant Cell Engineering.- 2.2.1 Protoplast Fusion.- 2.2.2 Cell Reconstruction Involving Fusion of Subprotoplasts.- 2.2.3 Cell Modification Through Uptake of Isolated Cell Organelles by Plant Protoplasts.- 2.3 Problems of Genetic Variability.- 2.3.1 Genetic Variability Associated with in Vitro Manipulation of Plant Cells.- 2.3.2 Genetic Changes in Cells Induced During the Stage of Isolated Protoplasts.- 3 Protoplast Fusion and Parasexual Hybridization of Higher Plants.- 3.1 Perspectives.- 3.1.1 Terminology.- 3.1.2 History of Parasexual Hybridization of Higher Plants.- 3.2 Methods of Screening for Parasexual Hybrids.- 3.2.1 Introduction.- 3.2.2 Genetic Complementation.- 3.2.3 Physiological Complementation.- 3.2.4 Restoration of Growth Capacity in Hybrids Upon Fusion of Inactivated Cells.- 3.2.5 Mechanical Isolation.- 3.2.6 Physical Enrichment.- 3.3 Frequency of Formation of Parasexual Hybrids.- 3.4 Protoplast Fusion and the Bypassing of Sexual Barriers.- 3.5 Analysis of the Parasexual Hybridization Process and the Genetic Composition of Plant Forms Arising from Protoplast Fusion.- 3.5.1 General Guidelines.- 3.5.2 Genetic Methods of Analysis.- Hybrid Crosses.- Cloning.- Cytogenetic Studies.- Cytophotometric Measurement of Nuclear DNA Content.- 3.5.3 Biochemical Analysis.- Multiple Molecular Forms of Enzymes.- Analysis of Fraction I Protein.- Restriction Endonuclease Analysis of Chloroplast and Mihondrial DNA.- Molecular Hybridization of Nucleic Acids.- Analysis of Low Molecular Weight Substances.- 4 Transmission Genetics of Parasexual Hybridization in Closely Related Crosses.- 4.1 General Considerations.- 4.1.1 Perspectives and Philosophy of Analysis of Transmission Genetics.- 4.1.2 Initial Experiments on Transmission Genetics of Parasexual Hybrids.- 4.1.3 Hybridization of More Than Two Parental Cells.- 4.2 Fate of Extranuclear Genetic Determinants.- 4.2.1 Perspectives.- 4.2.2 Protoplast Fusion and the Formation of Cytoplasmic Heterozygotes.- 4.2.3 Mitotic Segregation of Plasmagenes and the Sorting Out of Parental Genotypes.- 4.2.4 Recombination of Plasmagenes.- 4.3 Fate of Nuclear Genes in the Process of Somatic Hybridization.- 4.3.1 Segregation of Nuclei.- 4.3.2 Chromosome Sets of Hybrid Cells.- 4.4 Transmission Genetics in Hybridization Systems Utilizing Parental Cell Inactivation.- 4.4.1 Consideration of Animal and Bacterial Systems.- 4.4.2 Treatment with Iodoacetate.- 4.4.3 Irradiation.- 4.5 Genetic Diversity of Hybrid Plants Obtained by Protoplast Fusion.- 5 Protoplast Fusion and Hybridization of Distantly Related Plant Species.- 5.1 Introduction.- 5.2 Initial Stages of Culture of Hybrid Cells of Distantly Related Plant Species.- 5.3 Interfamily Cell Hybrids of Higher Plants.- 5.4 Intertribal Hybrids of Higher Plants.- 5.4.1 Hybridization of Cruciferae Species.- 5.4.2 Hybridization of Distantly Related Members of the Solanaceae and Other Families.- 5.4.3 General Conclusions of Results Obtained with Intertribal Hybrids of Higher Plants.- 5.5 Intergeneric Hybrids.- 5.6 Influence of Physical and Chemical Factors on the Fate of Genetic Material in Distantly Related Species Combinations.- 5.6.1 Introduction.- 5.6.2 Irradiation and the Induction of Genetic Asymmetry.- 5.6.3 Induction of Mitotic Chromosome Segregation by Chemical Agents.- 6 Use of Somatic Hybridization.- 6.1 Genetic Analysis Using Parasexual Hybridization.- 6.1.1 Introduction and Perspective.- 6.1.2 Analysis of the Nature of Inherited Traits.- 6.1.3 Analysis of Nuclear Genes.- Resistance to 5-Methyltryptophan.- Resistance to S-(2-Aminoethyl)-L-Cysteine.- Resistance to Cycloheximide.- Resistance to Azetidine-2-Carboxylate.- Additional Complementation Studies.- 6.1.4 Cosegregation of Extranuclear Genes.- 6.1.5 Analysis of Mitotic Cycle Mechanisms.- 6.1.6 Analysis of the Mechanisms of Differentiation and Morphogenesis.- 6.2 Practical Use of Somatic Cell Hybridization.- 6.2.1 Introduction.- 6.2.2 Bypassing of Incompatibility in Interspecific Hybridization.- 6.2.3 Reconstruction of Cytoplasmic Genomes.- 6.2.4 Transfer of Cytoplasmic Genomes and Cytoplasmic Male Sterility.- 7 Conclusion.- References.
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