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From The CriticsReviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This useful book is an excellent reference for a wide range of real-time PCR technologies and applications that also provides an overview of the theory of the increasingly important technique.
Purpose: The purpose is to provide both novice and experienced users with an invaluable reference to all of this technology.
Audience: The book is written to help readers at all levels to develop their own innovative approaches to real-time PCR.
Features: The initial chapters provide important information about real-time PCR, ranging from choosing an instrument and probe system to set-up, nucleic acid synthesis, sample extraction controls, validation, and data analysis. Subsequent chapters provide a comprehensive overview of important real-time methodologies such as quantification, expression analysis, and mutation detection. Concluding chapters deal with the application of real-time PCR to the diagnosis of infectious diseases, biodefence, veterinary science, food authenticity, and molecular haplotyping. The chapter on the validation of real-time PCR assays for infectious diseases provides essential information about the use of real-time PCR, a most widely used technique for the routine diagnosis of infectious diseases.
Assessment: This is an extremely useful book for both novice and experienced users that serves to stimulate readers at all levels to develop their own innovative approaches to real-time PCR.