Recombinant Proteins from Plants / Edition 1

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In Recombinant Proteins from Plants, leading researchers from around the world present the latest molecular and classical approaches to the separation, analysis, and characterization of recombinant plant proteins. Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future - such techniques as those for the efficient transformation of monocots with Agrobacterium tumifaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins." "Recombinant Proteins from Plants provides a comprehensive and detailed handbook of essential techniques that will benefit both scientists new to the field and established laboratories. It will become the standard reference for even highly skilled researchers working in this most exciting and fast developing field of biology.

" authoritative collection of basic and advanced protocols, providine clear, step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants."

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Editorial Reviews

A collection of basic and advanced protocols by which biotechnologists can express recombinant proteins using plants rather than single-cell fermentation systems. Provides step-by-step instructions for cloning, expressing, and analyze a wide variety of such proteins and for cloning genes in a number of different plant species. Also discusses characterizing medically important proteins expressed in plants, transforming monocots and dicots, and practical suggestions drawn from experience. The CiP data omits the subtitle. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780896033900
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/24/1997
  • Series: Methods in Biotechnology Series, #3
  • Edition description: 1998
  • Edition number: 1
  • Pages: 308
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.75 (d)

Table of Contents

Rice Transformation by Agrobacterium Infection, X. Cheng, R. K. Sardana, and I. Altosaar. Barley as a Producer of Heterologous Protein, L. Mannonen, K. Aspegren, A. Ritala, H. Simola, and T. H. Teeri. Introducing and Expressing Genes in Legumes, A. D. Bavage, M. P. Robbins, L. Skøt, and K. J. Webb. Use of the GUS Reporter Gene, J. Wilkinson and K. Lindsey. Expression of Recombinant Proteinase Inhibitors in Plants, D. Michaud and T. C. Vrain. Detection of Recombinant Viral Coat Protein in Transgenic Plants, H. Steinkellner and I. Korschineck. Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants, R. K. Sardana, P. R. Ganz, A. Dudani, E. S. Tackaberry, X. Cheng, and I. Altosaar. Tobamovirus Vectors for Expression of Recombinant Genes in Plants, T. H. Turpen and S. J. Reinl. Single-Chain Fv Antibodies Expressed in Plants, U. Conrad, U. Fiedler, O. Artsaenko, and J. Phillips. Characterization and Applications of Plant-Derived Recombinant Antibodies, R. Fischer, J. Drossard, Y. Liao, and S. Schillberg. Production of Recombinant Antibodiesin Plant Suspension Cultures, J. Drossard, Y.-C. Liao, and R. Fischer. Production of Foreign Proteins in Tobacco Cell Suspension Culture, V. Gomord, A.-C. Fitchette-Lainé, L.-A. Denmat, D. Michaud, and L. Faye. Transient Gene Expression in Plant Protoplasts, S. Schillberg, S. Zimmermann, D. Prüfer, D. Schuman, and R. Fischer. Stability of Recombinant Proteins in Plants, D. Michaud, T. C. Vrain, V. Gomord, and L. Faye. Trafficking and Stability of Heterologous Proteinsin Transgenic Plants, J. A. Napier, G. Richard, and P. R. Shewry. Screening for Transgenic Lines with Stableand Suitable Accumulation Levels of a Heterologous Protein, M. De Neve, H. Van Houdt, A.-M. Bruyns, M. Van Montagu, and A. Depicker. Manipulation of Photosynthetic Metabolism, M. A. J. Parry, S. P. Colliver, P. J. Madgwick, and M. J. Paul. Quantification of Heterologous Protein Levelsin Transgenic Plants by ELISA, A.-M. Bruyns, M. De Neve, G. De Jaeger, C. De Wilde, P. Rouzé, and A. Depicker. Analysis of N- and O-Glycosylation of Plant Proteins, A.-C. Fitchette-Lainé, L.-A. Denmat, P. Lerouge, and L. Faye. Two-Dimensional Electrophoresis: A Tool for Protein Separation and Processing for Microsequencing, N. P. E. Reddy and M. Jacobs. Index.

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