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Resin Microscopy and On-Section Immunocytochemistry / Edition 2

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Since antibodies tagged with markers have been developed, immunocyhemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.

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Editorial Reviews

From the Publisher

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohishemical laboratory. It extensively covers the field of onsection immunocyhemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling prools … . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. … it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocyhemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)

A look at the various techniques used in resin embedding as a method of preparing tissue samples for immunomicroscopic examination. For newcomers to the field, or practicing immunochemists who spaced out a journal or two last year and need to catch up, considers general theoretical and practical aspects, commercially available resins and their use, on-section single and double labeling methods, and other topics. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9783540672777
  • Publisher: Springer Berlin Heidelberg
  • Publication date: 5/18/2001
  • Series: Springer Lab Manuals Series
  • Format: Ringbound
  • Edition description: 2nd ed. 2001
  • Edition number: 2
  • Pages: 273
  • Product dimensions: 9.25 (w) x 7.50 (h) x 0.62 (d)

Table of Contents

Part I. Resin Embedding: Strategic Approach. The Resins. Resin Embedding Prools for Chemically Fixed Tissue. Cryotechniques. Methods for Resin Polymerisation: Handling Resin Blocks.- Part II. On-Section Immunolabelling: Strategies in Immunolabelling. General Considerations. Immunolabelling Prools for Resin Sections. Resin Embedding and Immunolabelling. List of Suppliers.
The complete table of contents can be found on the Internet:

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