Resin Microscopy and On-Section Immunocytochemistry

by Geoffrey R. Newman, Jan A. Hobot

Overview

Since antibodies tagged with markers have been developed, immunocyhemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cyhemistry and immunocyhemistry are described, including detailed prools for the application.

Product Details

ISBN-13:	9783540672777
Publisher:	Springer Berlin Heidelberg
Publication date:	04/10/2001
Series:	Springer Lab Manuals
Edition description:	Second Edition 2001
Pages:	273
Product dimensions:	8.27(w) x 10.98(h) x 0.02(d)

Table of Contents

I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Prools for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Prools for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I Examples of Typical Resin Embedding Regimes for Immunocyhemistry.- I.1 Solid Tissue, Pellets and Agar Blocks — Immersion Fixation.- I.2 Solid Tissue — Light Perfusion Fixation.- I.3 Solid Tissue — Very Light Perfusion Fixation.- I.4 Cryosubstitution.- I.5 Project Planner.- Appendix II List of Suppliers.- EM General.- EM Apparatus.- Electron Microscopes.- Flow Cytometry.- References.

Editorial Reviews

A look at the various techniques used in resin embedding as a method of preparing tissue samples for immunomicroscopic examination. For newcomers to the field, or practicing immunochemists who spaced out a journal or two last year and need to catch up, considers general theoretical and practical aspects, commercially available resins and their use, on-section single and double labeling methods, and other topics. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohishemical laboratory. It extensively covers the field of onsection immunocyhemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling prools … . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. … it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocyhemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)

From the Publisher