Resin Microscopy and On-Section Immunocytochemistry

Overview

Since antibodies tagged with markers have been developed, immunocyhemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the ...

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Overview

Since antibodies tagged with markers have been developed, immunocyhemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.

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Editorial Reviews

Booknews
A look at the various techniques used in resin embedding as a method of preparing tissue samples for immunomicroscopic examination. For newcomers to the field, or practicing immunochemists who spaced out a journal or two last year and need to catch up, considers general theoretical and practical aspects, commercially available resins and their use, on-section single and double labeling methods, and other topics. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohishemical laboratory. It extensively covers the field of onsection immunocyhemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling prools … . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. … it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocyhemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)

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Product Details

  • ISBN-13: 9783642477317
  • Publisher: Springer Berlin Heidelberg
  • Publication date: 9/12/2014
  • Series: Springer Lab Manuals
  • Edition description: 2nd ed. 2001
  • Edition number: 2
  • Pages: 273

Table of Contents

Pt. I Resin Embedding 1
1 The Strategic Approach 3
2 The Resins 34
3 Resin Embedding Protocols for Chemically Fixed Tissue 64
4 Cryotechniques 88
5 Methods for Resin Polymerisation 109
6 Handling Resin Blocks 130
Pt. II On-Section Immunolabelling 135
7 Strategies in Immunolabelling 137
8 General Considerations 164
9 Immunolabelling Protocols for Resin Sections 195
10 Resin Embedding and Immunolabelling 215
App. I Examples of Typical Resin Embedding Regimes for Immunocytochemistry 229
App. II: List of Suppliers 240
References 246
Index 265
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