RNA: Protein Interactions / Edition 1

RNA: Protein Interactions / Edition 1

by Christopher W. J. Smith
     
 

ISBN-10: 0199636508

ISBN-13: 9780199636501

Pub. Date: 07/28/1998

Publisher: Oxford University Press, USA

RNA-Protein Interactions: A Practical Approach provides a clear and co mprehensive guide to the many experimental procedures used in studying RNA-protein interactions. The approaches covered range from those ini tially used to detect a novel RNA-protein interaction to various bioch emical and genetic approaches, and from in-depth methods for analyzing the

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Overview

RNA-Protein Interactions: A Practical Approach provides a clear and co mprehensive guide to the many experimental procedures used in studying RNA-protein interactions. The approaches covered range from those ini tially used to detect a novel RNA-protein interaction to various bioch emical and genetic approaches, and from in-depth methods for analyzing the structural basis of the interaction to methods for purifying and cloning RNA binding proteins. The volume contains a number of procedur es that have not previously been covered in this type of manual, inclu ding the production of site-specifically modified RNAs by enzymatic an d chemical methods and in vivo screening for novel RNA-protein interac tions in yeast and E. coli. This informative book will be a valuable a id to laboratory workers in biochemistry and molecular biology, as wel l as graduate students and postdoctoral and senior scientists, whose r esearch encompasses this field.

Product Details

ISBN-13:
9780199636501
Publisher:
Oxford University Press, USA
Publication date:
07/28/1998
Series:
Practical Approach Series, #192
Edition description:
New Edition
Pages:
368
Product dimensions:
6.10(w) x 9.20(h) x 0.80(d)

Table of Contents

List of contributors
Abbreviations
1Applications of chemically synthesized RNA1
2Preparation of RNA: protein complexes for X-ray crystallography and NMR37
3Uses of site-specifically modified RNAs constructed by RNA ligation75
4The electrophoretic mobility shift assay for RNA binding proteins109
5Affinity methods for isolating RNA binding proteins137
6Synthetic lethal/enhancer screening to identify snRNA:protein and protein:protein interactions in yeast pre-mRNA splicing161
7Detecting RNA:protein interactions and isolating cDNA clones by Northwestern screening183
8A three-hybrid system to detect and analyse RNA:protein interactions in vivo195
9In vivo selection of specific RNA binding polypeptides using a transcription anti-termination reporter assay217
10Footprinting and modification - interference analysis of binding sites on RNA237
11Analysis of RNA:protein and RNA:RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques255
12In vitro selection of nucleic acid ligands285
A1. List of suppliers327
Index335

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