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Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned prools take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes prools for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Prools is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.
The book contains black-and-white illustrations.
Introduction to Isolating RNA
Donald E. Macfarlane and Christopher E. Dahle.
Large and Small Scale RNA Preparations from Eukaryotic Cells
Wolfgang Uckert, Wolfgang Walther, and Ulrike Stein.
An Improved Rapid Method of Isolating RNA from Cultured Cells
David B. Batt, Gordon G. Carmichael, and Zhong Liu.
Isolating RNA with the Cationic Surfactant, Catrimox-14
Christopher E. Dahle and Donald E. Macfarlane.
RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
Giorgio Stanta, Serena Bonin, and Rosella Perin.
Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides
Shu-Hua Cheng and Jeffrey R. Seemann.
Isolation of Plant Mihondrial RNA from Green Leaves
Fei Ye, Wolfgang O. Abel, and Ralf Reski.
Extraction from Fresh and Frozen Blood
Bimal D. M. Theophilus.
Isolation of Total RNA from Bacteria
Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel
Tapas Mukhopadhyay and Jack A. Roth.
Isolation of Messenger RNA
Sian Bryant and David L. Manning
Formaldehyde Gel Electrophoresis of Total RNA
Sian Bryant and David L. Manning.
UV Spectrophotometric Analysis of Ribonucleic Acids
Ralph Rapley and John Heptinstall.
Preparation of RNA Dot-Blots
Nonradioactive Northern Blotting
The Use of RNA Probes for the Analysis of Gene Expression
Analysis of RNA by Northern Blotting Using Riboprobes
Rai Ajit K. Srivastava.
RNA Quantitative Analysis form Fixed and Paraffin-Embedded Tissues
Giorgio Stanta, Serena Bonin, and René Utrera.
Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing
Anu Suomalainen and Ann-Christine Syvänen.
Preparation of Tissue Sections and Slides for mRNA Hybridization
Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes
Giorgio Terenghi .
One-Tube RT-PCR with Sequence-Specific Primers
Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA
Thomas C. G. Bosch and Jan U. Lohmann.
Characterization of RNA Using Continuous RT-PCR Coupled with ELISA
François Mallet, Guy Oriol, and Bernard Mandrand.
Gene Expression Analysis by CD-RT-PCR
Eric de Kant.
Primer Extension Analysis of mRNA
Maggie Walmsley, Mark Leonard, and Roger Patient.
S1 Mapping Using Single-Stranded DNA Probes
Stéphane Viville and Roberto Mantovani.
Measurements of Rate of Transcription in Isolated Nuclei by Nuclear 'Run-Off' Assay
Rai Ajit Srivastava and Gustav Schonfeld.
Transcription In Vitro Using Bacteriophage RNA Polymerases
Elaine T. Schenborn.
In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System
Louise Olliver and Charles D. Boyd.
In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
Louse Olliver, Ann Grobler-Rabie, and Charles D. Boyd.
The Xenopus Egg Extract Translation System
Glenn M. Matthews and Alan Colman.
Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization
Lesley-Ann Martin and Peter P. C. Mertens.