RNA Viruses : A Practical Approach / Edition 1

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Overview

RNA Viruses is wide ranging in scope, from core methods to emerging technology such as reverse genetics and retrovirus vectors. Chapter one covers the fundamentals of investigating RNA virus genome structure at a molecular level. Chapters two and three describe techniques for mutagenesis of RNA genomes and analysis of transcription. Chapter four deals with RNA virus-encoded proteinases, and important aspect of the control of RNA virus gene expression. Chapter five considers retrovirus oncogenesis and chapter six analysis of RNA virus quasispecies. Chapter seven describes systems for investigation of in vitro replication of positive-stranded viruses and chapter eight the packaging of RNA virus genomes. In addition to the technical aspects of reverse genetics and retrovirus vectors, both of the final two chapters also consider ethical aspects of these new technologies.

All protocols are provided in easy to follow step format with full listing of equipment and reagents required. These methods really work. To encourage success, the authors also list useful hints and tips, discuss potential problems and their solution and present typical data. No other methods manual in this field is as comprehensive in its support. The book is therefore highly recommended for all researchers who work on RNA viruses.

The book contains black-and-white illustrations.

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Editorial Reviews

Booknews
Details the major experimental methods currently employed by virologists, focusing on RNA in this volume. They range from emerging technology such as reverse genetics and retrovirus vectors, to money- saving tips such as making rather than buying silica particles for high-efficiency RNA extraction and liposomes for cell transfection. Companion volumes are alluded to, but only one on DNA viruses is specified. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780199637164
  • Publisher: Oxford University Press, USA
  • Publication date: 6/28/2000
  • Series: Practical Approach Series , #226
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 288
  • Product dimensions: 9.60 (w) x 7.40 (h) x 0.60 (d)

Table of Contents

List of protocols Abbreviations
1. Investigation of RNA virus genome structure, A.J. Easton, A.C. Marriott and C.R. Pringle
2. Mutagenesis of RNA virus genomes, W.S. Barclay and J.W. Almond
3. Analysis of transcriptional control in RNA virus infections, S. Makino
4. Analysis of RNA virus-encoded proteinases, M.D. Ryan, et al.
5. Detection and analysis of host gene targets for oncogenic retroviruses, J.C. Neil and A. Terry
6. Analysis of RNA virus quasispecies, J.K. Ball
7. In vitro replication of RNA viruses, R. Banerjee, et al.
8. Packaging of segmented and non-segmented RNA virus genomes, J. Barr and J.W. McCauley
9. RNA virus reverse genetics, A/ Bridgen and R.M. Elliott
10. Development of RNA virus vectors for gene delivery, B.A. Usmani, A. Fassati and G. Dickson
List of suppliers Index Investigation of RNA Virus Genome Structure Mutagenesis of RNA Virus Genomes Analysis of Transcriptional Control in RNA Virus Infections Analysis of RNA Virus-encoded Proteinases Detection and Analysis of Host Gene Targets for Oncogenic Retroviruses Analysis of RNA Virus Quasispecies In Vitro Replication of RNA Viruses Packaging of Segmented and non-segmented RNA Virus Genomes RNA Virus Reverse Genetics Development of RNA Virus Vectors for Gene Delivery

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