RNAi: A Guide to Gene Silencing / Edition 2

by Gregory J. Hannon

Overview

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

Product Details

ISBN-13:	9780879697044
Publisher:	Cold Spring Harbor Laboratory Press
Publication date:	08/01/2003
Pages:	448
Product dimensions:	8.50(w) x 10.90(h) x 0.90(d)

Table of Contents

Chapter 1. Sense Cosuppression in Plants: Past, Present, and Future
Richard A. Jorgensen
Chapter 2. Transgene Cosuppression in Animals
James A. Birchler, Manika Pal–Bhadra, and Utpal Bhadra*
Chapter 3. Regulation of the Genome by Double–stranded RNA
Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke
Chapter 4. RNAi in Caenorhabditis elegans
René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
Chapter 5. Biochemistry of RNAi in Drosophila
G. Hannon and P. Zamore
Chapter 6. Stable Suppression of RNAi in Mammals
D. Conklin, P. Sharp, and M. McManus
Chapter 7. RNAi Proteins and Functions in Plants: Genetics of RNAiin Plants
R. Martienssen
Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing
Allen W. Nicholson
Chapter 9. RNA–dependent RNA Polymerase in Gene Silencing
Luyun Huang, John Gledhill, and Craig E. Cameron
Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization
Ira M. Hall and Shiv I.S. Grewal
Chapter 11. Total Interference: Genome–wide RNAi Screens in C. elegans
Andrew G. Fraser and Julie Ahringer
Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants
Tessa M. Burch–Smith, Jennifer L. Miller, and S.P. Dinesh–Kumar
Chapter 13. Mammalian RNA Interference
Thomas Tuschl
Chapter 14. RNAi in Avian Embryos
Esther T. Stoeckli
Chapter 15.
Guide to RNAi in Mouse Oocytes and Preimplantation Embryos
Paula Stein and Petr Svoboda
Chapter 16. RNAi Technologies in Drosophila Cell Culture
Scott M. Hammond
Chapter 17. RNAi Applications in Drosophila melanogaster
Richard W. Carthew
Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms
Elisabetta Ullu and Christian Tschudi