Septic Shock Methods and Protocols / Edition 1

by Thomas J. Evans

Overview

Septic shock remains a serious medical condition with high mortality. Despite many advances in intensive care medicine and antibiotic devel- ment, this has not changed appreciably in the last 20 years. Frustratingly, over the same period of time, enormous advances have been made in understa- ing the underlying pathogenic mechanisms of this condition. This has resulted in the development of several novel therapies for septic shock, which, despite excellent theoretical grounds for their efficacy, have failed in altering mort- ity attributable to sepsis. The reasons for these failures are multiple, but it is clear that further research is required aimed at increasing our understanding of the basic pat- physiological processes that occur following infection. Research into septic shock draws upon a number of different disciplines, ranging from molecular and cellular biology to physiological measurements on whole animals. Septic Shock Methods and Prools is an attempt to draw together into one volume a number of prools that are of use in the investigation of the mechanisms of septic shock. I have divided the book into five sections. The first deals with endotoxin, the lipopolysaccharide component of the Gram-negative cell m- brane that can mimic many of the features of septic shock. Gram-positive organisms are found increasingly as causes of septic shock, and several - pects of toxins produced from these bacteria are considered in the second s- tion.

Product Details

ISBN-13:	9781617371547
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Methods in Molecular Medicine , #36
Edition description:	Softcover reprint of hardcover 1st ed. 2000
Pages:	208
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

About the Author

Evans, Thomas J. (Imperial Coll School of Medicine)

Table of Contents

Endotoxin.- Assay of Endotoxin by Limulus Amebocyte Lysate.- Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria.- Assay of Anti-Endotoxin Antibodies.- Isolation of the Bactericidal/Permeability-Increasing Protein from Polymorphonuclear Leukocytes by Reversible Binding to Target Bacteria.- Purification of Lipopolysaccharide Binding Protein.- Other Bacterial Products.- Purification of Strepoccal Pyrogenic Exotoxin A.- Assay for Superantigens.- Cytokines.- Bioassay for Tumor Necrosis Factors-? and—.- Assay of Soluble Tumor Necrosis Factor Receptors.- Whole-Blood Assays for Cytokine Production.- Nitric Oxide and Other Reactive Nitrogen Intermediates.- Nitric Oxide Synthase Inhibitors.- In Situ Detection of Nitric Oxide.- Immunochemical Detection of Nitric Oxide Synthase in Human Tissue.- Anti-Nitrotyrosine Antibodies for Immunohishemistry.- Detection of Peroxynitrite in Biological Fluids.- Cell Culture Techniques.- Myocardial Cells in Culture to Study Effects of Cytokines.- Culture of Primary Human Bronchial Epithelial Cells.- Primary Culture of Human Proximal Renal Tubular Epithelial Cells.

Editorial Reviews

"this text is a fine laboratory reference. . . the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies. "-Doody's Health Sciences Book Review Journal

From the Publisher

3 Stars from Doody

This is a collection of laboratory procedures for investigating the mechanisms of septic shock. Over 30 international investigators have made contributions to this volume on topics ranging from purification of endotoxin to the immunochemical detection of nitric oxide synthase. The text is written as a laboratory manual for investigators engaged in septic shock research. The text has a multidisciplinary appeal to a broad range of scientific investigators in medicine and surgery. Any investigator or student engaged in clinical or experimental studies of the pathophysiologic processes associated with the septic process following infection will benefit from this book. The text has been organized around five separate subject areas: analysis and purification of endotoxin; purification and analysis of streptococcal exotoxin and superantigens; analysis of selected cytokines; analysis of nitric oxide and other oxidant/nitrating agents; and selected cell culture techniques. While the myriad in vitro or in situ techniques for investigating septic shock are not chronicled here, the five areas mentioned are adequately covered, so this would be a useful laboratory reference. I believe that this text is a fine laboratory reference. The section on tissue culture is a bit superficial but it would be difficult, given the space allowed, to include all of the various tissue culture methodologies currently available. Overall, the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators andstudents engaged in septic shock/infection studies.

Charles E. Edmiston

Specialists in a range of disciplines from molecular and cellular biology to the physiological measurement of entire animals describe proven methods for investigating the often deadly mechanisms of septic shock. They consider sepsis involving endotoxins, toxins derived from Gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and primary cell cultures. They also treat a number of significant techniques that are not widely described in the literature, including the endotoxin assay, the bioassay of TNR, purifying bacterial endotoxin and superantigens, and culturing primary human cells. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Reviewer: Charles E. Edmiston, Jr., PhD (Medical College of Wisconsin)
Description: This is a collection of laboratory procedures for investigating the mechanisms of septic shock. Over 30 international investigators have made contributions to this volume on topics ranging from purification of endotoxin to the immunochemical detection of nitric oxide synthase.
Purpose: The text is written as a laboratory manual for investigators engaged in septic shock research. The text has a multidisciplinary appeal to a broad range of scientific investigators in medicine and surgery.
Audience: Any investigator or student engaged in clinical or experimental studies of the pathophysiologic processes associated with the septic process following infection will benefit from this book.
Features: The text has been organized around five separate subject areas: analysis and purification of endotoxin; purification and analysis of streptococcal exotoxin and superantigens; analysis of selected cytokines; analysis of nitric oxide and other oxidant/nitrating agents; and selected cell culture techniques. While the myriad in vitro or in situ techniques for investigating septic shock are not chronicled here, the five areas mentioned are adequately covered, so this would be a useful laboratory reference.
Assessment: I believe that this text is a fine laboratory reference. The section on tissue culture is a bit superficial but it would be difficult, given the space allowed, to include all of the various tissue culture methodologies currently available. Overall, the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies.

Doody's Review Service