Sexually Transmitted Diseases / Edition 1by Rosanna Peeling
Pub. Date: 11/09/2010
Publisher: Springer-Verlag New York, LLC
In Sexually Transmitted Diseases: Methods and Protocols, Rosanna Peeling and P. Frederick Sparling have created a comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of sexually transmitted diseases, including HIV. The methods-each crafted by a leading expert for comprehensiveness and genuine laboratory… See more details below
In Sexually Transmitted Diseases: Methods and Protocols, Rosanna Peeling and P. Frederick Sparling have created a comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of sexually transmitted diseases, including HIV. The methods-each crafted by a leading expert for comprehensiveness and genuine laboratory utility-cover the major sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes simplex. Additional molecular techniques are included for detecting the genital mycoplasmas, hepatitis B virus, HIV-1, human papillomavirus.
This book will significantly enhance our ability to detect sexually transmitted infections and to conduct research to further our understanding of sexually transmitted diseases. Eminently practical and up-to-date, Sexually Transmitted Disease Protocols provides a comprehensive collection of reliable molecular methods for STD diagnosis, research, and patient management.
Table of Contents
Part I. Overview.
The Impact of Molecular Technology on STD Control: A Historical Perspective
P. Frederick Sparling
Part II. Molecular Techniques for the Detection of Sexually Transmitted Pathogens.
Neisseria gonorrhoeae: Detection and Typing by Probe Hybridization, LCR, and PCR
Charlotte A. Gaydos and Thomas C. Quinn
Molecular Diagnosis for Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR, TMA, and Q-ß Replicase
Max A. Chernesky and James B. Mahony
Haemophilus ducreyi Detection by PCR
Patricia A. Totten, Jane Kuypers, and Stephen A. Morse
Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR
Karina A. Orle and Judith B. Weiss
Detection of Genital Mycoplasmas by PCR
Claire B. Gilroy and David Taylor-Robinson
Hepatitis B Virus: Detection and Quantitation by Membrane and Liquid Hybridization, Branched DNA Signal Amplification, Hybrid Capture, and PCR Methods
Molecular Diagnosis of HIV-1 by PCR
Susan A. Fiscus
Part III. Research Techniques for Epidemiology and the Management of STDs.
Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR
Ian W. Maclean
Genotyping Chlamydia trachomatis by PCR,Deborah Dean. Human Papillomavirus Detection by PCR and Typing by Dot-Blot
Agnetha Josefsson, Patrik Magnusson, and Ulf Gyllensten
Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring
Sharon A. Cassol, Francisco Diaz-Mitoma, and D. William Cameron
Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR
Susan A. Ficus and Myron S. Cohen
Part IV. Molecular Technology for STD Diagnosis and Research: The New Challenges.
Molecular Techniques for HIV and STDs: Implications for Research and Disease Control in the New Millenium
Rosanna W. Peeling, David C. W. Mabey, and King K. Holmes
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