Sexually Transmitted Diseases / Edition 1

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Overview

Provides the molecular methods for the detection and study of sexually transmitted diseases. Also includes an overview of the impact molecular biology has had on the ability to detect and study STDs followed by an analysis of the challenges facing those doing research in this extremely important field. The techniques are all provided in a step-by-step fashion and are supported by helpful Notes containing tricks of the trade and troubleshooting advice that ensure the technique's reproducibility by both experts or novices in the lab.

The book contains black-and-white illustrations.

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Editorial Reviews

Charles E. Edmiston
This is an excellent addition to the Humana Press series, "Methods in Molecular Medicine." The text is meant as a manual for STD investigators, covering all aspects of the molecular protocols for both laboratory diagnosis and investigating the epidemiology and management of sexually transmitted diseases. It describes several molecular techniques such as probe hybridization, LCR, PCR, pulse-field techniques for genotyping, and techniques for quantitative viral (HIV- 1) loads. This manual should be viewed as a primary resource encompassing diagnostic techniques currently used by prominent STD investigators and clinical laboratories specializing in the recovery of these microbial pathogens. The audience for this manual should include: clinical microbiologists engaged in molecular diagnostic studies; independent STD investigators; graduate students; epidemiologists engaged in molecular studies of STD transmission; and novice clinical scientists who require a well documented authoritative resource of the application of molecular techniques in STD research. The organization of this text is similar to other manuals in the Humana Press series, beginning with a general outline of the topic, discussion of specific molecular techniques, and a concise material and methods section. The discussion at the end of each chapter is supplemented with a "notes section" that the contributors have included to help the reader through the pitfalls often encountered when learning or applying new laboratory techniques. An extensive and recent literature citation is available at the end of each chapter. It is of importance to note that the techniques discussed in the various chapters often include a widerange of clinical samples such as swab, tissue, and body fluid analysis. This is a highly valuable and unique text since it describes molecular techniques for a wide range of STD pathogens (for example, Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilus ducreyi, Treponema pallidum, HBV, and HIV- 1). Fifty percent of the text is dedicated to describing diagnostic techniques currently used in the clinical or research laboratory. Five chapters have also been included that discuss methods appropriate for epidemiologic studies. The credibility of the text is greatly enhanced by the outstanding group of international STD investigators, which the authors have assembled for the purpose of developing a concise yet authoritative laboratory resource. This text would be a valuable acquisition for both STD investigators and students contemplating laboratory or clinical studies in this area of medicine.
Booknews
Comprises 14 contributions on molecular protocols for the laboratory diagnosis and study of STDs, with coverage of the major sexually transmitted pathogens including Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes simplex. Methods covered include probe hybridization, PCR, LCR, TMA, pulse field gel electrophoresis, and NASBA. Of interest to clinical microbiologists, virologists, infectious disease specialists, and epidemiologists. Annotation c. by Book News, Inc., Portland, Or.
Doody's Review Service
Reviewer: Charles E. Edmiston, Jr., PhD (Medical College of Wisconsin)
Description: This is an excellent addition to the Humana Press series, "Methods in Molecular Medicine." The text is meant as a manual for STD investigators, covering all aspects of the molecular protocols for both laboratory diagnosis and investigating the epidemiology and management of sexually transmitted diseases.
Purpose: It describes several molecular techniques such as probe hybridization, LCR, PCR, pulse-field techniques for genotyping, and techniques for quantitative viral (HIV- 1) loads. This manual should be viewed as a primary resource encompassing diagnostic techniques currently used by prominent STD investigators and clinical laboratories specializing in the recovery of these microbial pathogens.
Audience: The audience for this manual should include: clinical microbiologists engaged in molecular diagnostic studies; independent STD investigators; graduate students; epidemiologists engaged in molecular studies of STD transmission; and novice clinical scientists who require a well documented authoritative resource of the application of molecular techniques in STD research.
Features: The organization of this text is similar to other manuals in the Humana Press series, beginning with a general outline of the topic, discussion of specific molecular techniques, and a concise material and methods section. The discussion at the end of each chapter is supplemented with a "notes section" that the contributors have included to help the reader through the pitfalls often encountered when learning or applying new laboratory techniques. An extensive and recent literature citation is available at the end of each chapter. It is of importance to note that the techniques discussed in the various chapters often include a wide range of clinical samples such as swab, tissue, and body fluid analysis.
Assessment: This is a highly valuable and unique text since it describes molecular techniques for a wide range of STD pathogens (for example, Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilus ducreyi, Treponema pallidum, HBV, and HIV- 1). Fifty percent of the text is dedicated to describing diagnostic techniques currently used in the clinical or research laboratory. Five chapters have also been included that discuss methods appropriate for epidemiologic studies. The credibility of the text is greatly enhanced by the outstanding group of international STD investigators, which the authors have assembled for the purpose of developing a concise yet authoritative laboratory resource. This text would be a valuable acquisition for both STD investigators and students contemplating laboratory or clinical studies in this area of medicine.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9781617370649
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/9/2010
  • Series: Methods in Molecular Medicine Series , #20
  • Edition description: Softcover reprint of hardcover 1st ed. 1999
  • Edition number: 1
  • Pages: 244
  • Product dimensions: 0.58 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Part I. Overview.

The Impact of Molecular Technology on STD Control: A Historical Perspective

P. Frederick Sparling

Part II. Molecular Techniques for the Detection of Sexually Transmitted Pathogens.

Neisseria gonorrhoeae: Detection and Typing by Probe Hybridization, LCR, and PCR

Charlotte A. Gaydos and Thomas C. Quinn

Molecular Diagnosis for Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR, TMA, and Q-ß Replicase

Max A. Chernesky and James B. Mahony

Haemophilus ducreyi Detection by PCR

Patricia A. Totten, Jane Kuypers, and Stephen A. Morse

Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR

Karina A. Orle and Judith B. Weiss

Detection of Genital Mycoplasmas by PCR

Claire B. Gilroy and David Taylor-Robinson

Hepatitis B Virus: Detection and Quantitation by Membrane and Liquid Hybridization, Branched DNA Signal Amplification, Hybrid Capture, and PCR Methods

Mel Krajden

Molecular Diagnosis of HIV-1 by PCR

Susan A. Fiscus

Part III. Research Techniques for Epidemiology and the Management of STDs.

Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR

Ian W. Maclean

Genotyping Chlamydia trachomatis by PCR,Deborah Dean. Human Papillomavirus Detection by PCR and Typing by Dot-Blot

Agnetha Josefsson, Patrik Magnusson, and Ulf Gyllensten

Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring

Sharon A. Cassol, Francisco Diaz-Mitoma, and D. William Cameron

Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR

Susan A. Ficus and Myron S. Cohen

Part IV. Molecular Technology for STD Diagnosis and Research: The New Challenges.

Molecular Techniques for HIV and STDs: Implications for Research and Disease Control in the New Millenium

Rosanna W. Peeling, David C. W. Mabey, and King K. Holmes

Index

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