Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology / Edition 3

Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology / Edition 3

by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore
     
 

Providing condensed descriptions of more than 600 methods compiled from CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, this updated 4th Edition of the classic laboratory manual throoughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative… See more details below

Overview

Providing condensed descriptions of more than 600 methods compiled from CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, this updated 4th Edition of the classic laboratory manual throoughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated--SHORT PROTOCOLSoffers both.

The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used ein every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists...

THIS EDITION HAS BEEN EXPANDED TO INCLUDE:

  • Fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using BLAST.
  • Informatics for molecular biologists
  • Analysis of protein interactions
  • Epitope tagging
  • Mathematics and statistics for molecular biologists

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Product Details

ISBN-13:
9780471137818
Publisher:
Wiley
Publication date:
10/28/1995
Edition description:
Older Edition
Pages:
900
Product dimensions:
9.32(w) x 11.14(h) x 1.77(d)

Table of Contents

Escherichia Coli, Plasmids, and Bacteriophages.
Preparation and Analysis of DNA.
Enzymatic Manipulation of DNA and RNA.
Preparation and Analysis of RNA.
Construction of Recombinant DNA Libraries.
Screening of Recombinant DNA Libraries.
DNA Sequencing.
Mutagenesis of Cloned DNA.
Introduction of DNA into Mammalian Cells.
Analysis of Proteins.
Immunology.
DNA-Protein Interactions.
Saccharomyces Cerevisiae.
In Situ Hybridization and Immunohistochemistry.
The Polymerase Chain Reaction.
Protein Expression.
Analysis of Protein Phosphorylation.
Sequence Similarity Searching Using the Blast Family of Programs.
Analysis of Protein Interactions.
Appendices.
Index.

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