Short Protocols in Molecular Biology / Edition 2 by Frederick M. Ausubel | 9780471577355 | Paperback | Barnes & Noble
Short Protocols in Molecular Biology / Edition 2

Short Protocols in Molecular Biology / Edition 2

by Frederick M. Ausubel
     
 

ISBN-10: 0471577359

ISBN-13: 9780471577355

Pub. Date: 05/12/1992

Publisher: Wiley

Presents shortened versions of the methods published in Current Protocols in Molecular Biology (CPMB). Includes step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides sufficient detail to perform experiments. Cross-referenced to CPMB. Explains the hows and whys of many steps. Provides tips on safety, storage and

Overview

Presents shortened versions of the methods published in Current Protocols in Molecular Biology (CPMB). Includes step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides sufficient detail to perform experiments. Cross-referenced to CPMB. Explains the hows and whys of many steps. Provides tips on safety, storage and anticipated results. Includes references and recipes for all reagents and media.

Product Details

ISBN-13:
9780471577355
Publisher:
Wiley
Publication date:
05/12/1992
Edition description:
2nd ed
Pages:
740
Product dimensions:
7.95(w) x 10.07(h) x 1.68(d)

Table of Contents

Escherichia Coli, Plasmids, and Bacteriophages.
Preparation and Analysis of DNA.
Enzymatic Manipulation of DNA and RNA.
Preparation and Analysis of RNA.
Construction of Recombinant DNA Libraries.
Screening of Recombinant DNA Libraries.
DNA Sequencing.
Mutagenesis of Cloned DNA.
Introduction of DNA into Mammalian Cells.
Analysis of Proteins.
Immunology.
DNA-Protein Interactions.
Saccharomyces Cerevisiae.
In Situ Hybridization and Immunohistochemistry.
The Polymerase Chain Reaction.
Protein Expression.
Analysis of Protein Phosphorylation.
Sequence Similarity Searching Using the Blast Family of Programs.
Analysis of Protein Interactions.
Appendices.
Index.

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