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This book describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Such techniques form the basis of routine tests in chemical, biological, and medical science laboratories. Practical advice is given, from experts in the different topics, together with lists of useful literature.
The book contains black-and-white illustrations.
List of protocols Abbreviations
1. Introduction to light absorption; visible and ultraviolet spectra, Robert K. Poole and Uldis Kalnenieks
2. Fluorescence principles and measurement, Arthur G. Szabo
3. Time resolved fluorescence and phosphorescence spectroscopy, Thomas D. Bradrick and Jorge E. Churchich
4. An introduction to circular dichroism, Alison Rodger and Matthew A. Ismail
5. Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods, Wlodzimierz Bujalowski and Maria J. Jezewska
6. Steady state kinetics, Athel Cornish-Bowden
7. Spectrophotometric assays, T.J. Mantle and D.A. Harris
8. Stopped flow spectroscopy, M.T. Wilson and J. Torres
9. Stopped flow fluorescence spectroscopy, Michael G. Gore and Stephen P. Bottomley
10. Stopped flow circular dichroism, Alison Rodger and Michael J. Carey
11. Spectrophotometry and fluorimetry of cellular compartments and intracellular processes, C. Lindsay Bashford
12. Use of optical spectroscopic methods to study the thermodynamic stability of proteins, Maurice R. Eftink and Haripada Maity
13. The use of spectroscopic techniques in the study of DNA stability, John SantaLucia, Jr.
List of suppliers Index