Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques / Edition 1

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Overview

Although spectroscopic methods are of great use to biological scientists, many researchers lack a specialist's knowledge of these techniques. Spectroscopic Methods and Analyses provides hands-on information on these valuable research tools, emphasizing practical aspects, such as amounts and purity of required samples, limitations and advantages of the technique, and interpretation of the results, thus enabling every molecular biologist-either independently or with the help of a specialist-to evaluate the usefulness of a technique, to plan an original program, and to gain a realistic idea of the resources needed.An indispensable guide for all biochemists, cell and molecular biologists, pharmacologists, immunologists, pathologists, and neuroscientists.

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Editorial Reviews

From the Publisher
The main strength of this volume...is the extensive attention paid to the pragmatic and mundane issues that are only gleaned with difficulty from the literature, yet are absolutely critical for success. Those contemplating initiating studies employing these physical methods would be well served by consulting this manual. - Modern Pathology
Booknews
Although spectroscopic methods are of great use to biological scientists, many researchers lack a specialist's knowledge of these techniques. This guide provides hands-on information on these research tools, emphasizing practical aspects such as amounts and purity of required samples, limitations and advantages of the technique, and interpretation of the results, thus enabling molecular biologists--either independently or with the help of a specialist--to evaluate the usefulness of a technique, to plan an original program, and to gain a realistic idea of the resources needed. Plastic comb-binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780896032156
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 5/23/1993
  • Series: Methods in Molecular Biology Series , #17
  • Edition description: 1993
  • Edition number: 1
  • Pages: 395
  • Product dimensions: 8.27 (w) x 5.83 (h) x 0.92 (d)

Table of Contents

NMR Spectroscopy: Introduction to Nuclear Magnetic Resonance. Structural Studies of Proteins in Solution Using Proton Nuclear Magnetic Resonance. Peptide Structure Determination by NMR. High-Resolution NMR of DNA and Drug-DNA Interactions. Structural Characterization of the Carbohydrate Moieties of Glycoproteins by High-Resolution 1H-NMR Spectroscopy. The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides. Dynamic and Exchange Processes in Macromolecules Studied by NMR Spectroscopy. Mass Spectrometry: Introduction to Mass Spectrometry. Laser Desorption Ionization Mass Spectrometry of Bioorganic Molecules. 252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins. Fast Atom Bombardment Mass Spectrometry of Peptides. Tandem Mass Spectrometry. Metalloprotein Techniques: Mössbauer Spectroscopy. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins. Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation. The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins. Index.

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