Suicide Gene Therapy: Methods and Reviews / Edition 1

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The first comprehensive review of both the theory and practice of suicide gene therapy. The authors cover all the major aspects of suicide gene therapy, including the design and use of vectors in gene transduction, various enzyme and prodrug systems, the mechanistic analysis of the bystander effect, the design and synthesis of prodrugs, immunological implications, and its clinical impact. They also describe all the cutting-edge methods needed to explore, study, and advance understanding of the basic biology underlying gene therapy. Each fully tested method includes step-by-step instructions, a discussion of the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and notes on the interpretation and use of results.
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Editorial Reviews

Doody's Review Service
Reviewer: David A. Dean, Ph.D.(Northwestern University Feinberg School of Medicine)
Description: This book provides a background on the theory and some of the methods used to develop and evaluate current and developing approaches for suicide gene therapy. A variety of gene delivery strategies are discussed, as are mechanisms of drug action, host response, and clinical trial development.
Purpose: The purpose of the book is to provide a comprehensive overview of the theory and practices of suicide gene therapy to treat cancer. The book takes a multidisciplinary approach to integrate theory and practice. Suicide gene therapy has great potential importance for cancer treatment and thus a book like this is a welcome addition to the field.
Audience: This book is written for researchers at all levels, from graduate students in biological or pharmaceutical sciences to researchers and clinicians. As with any edited book, some chapters stand stronger than others, but on the whole it is accessible at all levels and is an appropriate resource for all as well. The authors are leaders in their fields, making this a credible contribution.
Features: This book is divided into several general areas. The first several chapters deal with different gene and pro-drug delivery methods, including viral and non-viral methods for gene delivery. The next set of chapters deals with specific combinations of enzymes and drugs to be used for suicide gene therapy. Finally, several chapters are included on such topics as immunological responses, how to design a clinical trial, and the various side effects that may be encountered. Notable chapters include one on the development of retroviral vectors by Colin Porter and excellent chapter on random and directed mutagenesis techniques and considerations by Kurtz and Black. The book contains a large number of high quality color and black-and-white illustrations that support and enhance the text. The only shortcoming of the book is that while the introductions and discussions of theory are excellent, in many instances the desire to add procedures in a protocol format takes away from the strengths of the book. Several chapters provide detailed protocols that are not generally applicable and thus, not of much use to people outside that particular laboratory. However, these are a relatively few number of chapters, and the overall tone of the book is very good.
Assessment: This is a good book for those working in the area of suicide gene therapy that can serve both as a reference to senior investigators as well as a primer to new lab members and researchers outside the field who are trying to get a flavor of the state of the field.

3 Stars from Doody
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Product Details

  • ISBN-13: 9780896039711
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/4/2003
  • Series: Methods in Molecular Medicine Series , #90
  • Edition description: 2004
  • Edition number: 1
  • Pages: 560
  • Product dimensions: 1.44 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Introduction to the Background, Principles, and State of the Art in Suicide Gene Therapy

Ion Niculescu-Duvaz and Caroline J. Springer

Introduction to Vectors for Suicide Gene Therapy

Caroline J. Springer

Construction of VNP20009: A Novel, Genetically Stable Antibiotic-Sensitive Strain of Tumor-Targeting Salmonella for Parenteral Administration in Humans

Kenneth Brooks Low, Martina Ittensohn, Xiang Luo, Li-Mou Zheng, Ivan King, John M. Pawelek, and David Bermudes

Nonreplicating DNA Viral Vectors for Suicide Gene Therapy: The Adenoviral Vectors

Masato Yamamoto and David T. Curiel

Replication-Selective Oncolytic Adenoviruses

Gunnel Halldén, Stephen H. Thorne, Jingping Yang, and David H. Kirn

Retroviral Vectors for Suicide Gene Therapy

Colin Porter

Nonviral Liposomes

Andrew D. Miller

Peptide- and Polymer-Based Gene Delivery Vehicles

Richard Brokx and Jean Gariépy

Design of Prodrugs for Suicide Gene Therapy

Dan Niculescu-Duvaz, Ion Niculescu-Duvaz, and Caroline J. Springer

Cyhrome P450-Based Gene Therapies for Cancer

E. Antonio Chiocca and David J. Waxman

Tumor Sensitization to Purine Analogs by E. coli PNP

Kimberly V. Curlee, William B. Parker, and Eric J. Sorscher

Enzyme-Prodrug Systems: Carboxylesterase/CPT-11

Mary K. Danks and Philip M. Potter

Enzyme-Prodrug Systems: Thymidine Phosphorylase/5'-Deoxy-5-Fluorouridine

Alexandre Evrard, Joseph Ciccolini, Pierre Cuq, and Jean-Paul Cano

Methods to Improve Efficacy in Suicide Gene Therapy Approaches: Targeting Prodrug-Activating Enzymes Carboxypeptidase G2 and Nitroreductase to Different Subcellular Compartments

Silke Schepelmann, Robert Spooner, Frank Friedlos, and Richard Marais

Extracellular b-Glucuronidase for Gene-Directed Enzyme-Prodrug Therapy

Sabine Brüsselbach

Enhancement of Suicide Gene Prodrug Activation by Random Mutagenesis

Jean-Emmanuel Kurtz and Margaret E. Black

Combination Suicide Gene Therapy

Wolfgang Uckert, Brian Salmons, Christian Beltinger, Walter H. Günzburg, and Thomas Kammertöns

Immune Response to Suicide Gene Therapy

Shigeki Kuriyama, Hirohisa Tsujinoue, and Hitoshi Yoshiji

Targeting Cancer With Gene Therapy Using Hypoxia as a Stimulus

Gabi U. Dachs, Olga Greco, and Gillian M. Tozer

Radiation-Activated Antitumor Vectors

Simon D. Scott and Brian Marples

In Vitro and In Vivo Models for the Evaluation of GDEPT: Quantifying Bystander Killing in Cell Cultures and Tumors

William R. Wilson, Susan M. Pullen, Alison Hogg, Stephen M. Hobbs, Frederik B. Pruijn, and Kevin O. Hicks

Suicide Gene Therapy in Liver Tumors

Long R. Jiao, Roman Havlik, Joanna Nicholls, Steen Lindkaer Jensen, and Nagy A. Habib

Clinical Trials With GDEPT: Cytosine Deaminase and 5-Fluorocytosine

Nicola L. Brown and Nicholas R. Lemoine

The Nitroreductase/CB1954 Enzyme-Prodrug System

Nicola K. Green, David J. Kerr, Vivien Mautner, Peter A. Harris, and Peter F. Searle

Side Effects of Suicide Gene Therapy

Marjolijn M. van der Eb, Bertie de Leeuw, Alex J. van der Eb, and Rob C. Hoeben

Antibody-Directed Enzyme-Prodrug Therapy

R. Barbara Pedley, Surinder K. Sharma, Robert E. Hawkins, and Kerry A. Chester

Bioreductive Prodrugs for Cancer Therapy

Beatrice Seddon, Lloyd R. Kelland, and Paul Workman


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