Systematic Approach to Evaluation of Mouse Mutations

by John P. Sundberg (Editor)

Overview

Experts from The Jackson Laboratory and around the world provide practical advice on everything from how to establish a colony to where to go for specific mutations. The book includes information on medical photography, grafting procedures, and how to map the genes and evaluate the special biological characteristics of mice. It also discusses how to maintain a colony of mice that breed with difficulty, how to approach mapping spontaneous mutations, how to set up systems to evaluate a specific antibody, how to perform simple measurements that yield a large amount of information, and how to access mouse informatics on the Web.

Product Details

ISBN-13:	9781040202357
Publisher:	CRC Press
Publication date:	07/23/1999
Sold by:	Barnes & Noble
Format:	eBook
Pages:	216
File size:	1 MB

About the Author

John P. Sundberg, Dawnalyn Boggess

Table of Contents

Mouse Colony Establishment-Determining the Genetic Basis of a New Trait-Xavier Montagutelli Computerized Colony Record Keeping-Medical Record Keeping for Project Analysis-Necropsy Methods for Laboratory Mice. Biological Characterization of a New Mutation- Photography of Mutant Laboratory Mice-Comparative Pathology and Animal Model Development-Kinetics and Morphometrics-Ultrastructural Evaluation of Mouse Mutations-Immunohistochemical and Immunofluoresence Methods-Radiolabeled cRNA and In Situ Hybridization-In Situ Hybridization with Non-Radiolabeled Probes-Repositories of Mouse Mutations and Inbred, Congenic and Recombitant Inbred Strains

Editorial Reviews

For researchers working with the most widely used animal model for studying mammals, specialists from the Jackson Laboratory in Maine and elsewhere describe how to identify a new mouse mutation, establish a colony, map the gene, evaluate the special biological characteristics of mice, and determine how they may be used. Most of the methods they describe are essentially scaled-down versions of standard operating procedures for diagnostic medicine and biomedical research. Some of the matters they touch on are medical record keeping, photography, kinetics and morphometrics, ultrastructure evaluation, and radiolabled cRNA for hybridization. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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