Techniques and Protocols in Molecular Biology

by Elsevier Science

Overview

Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.

Product Details

ISBN-13:	9780443141607
Publisher:	Elsevier Science
Publication date:	11/01/2025
Pages:	250
Product dimensions:	7.50(w) x 9.25(h) x 0.00(d)

About the Author

Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.

Table of Contents

1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria
2. Isolation of RNA
3. PCR (Polymerase Chain reaction)
4. Real-time (Quantitative) PCR
5. Electrophoresis
6. 3' RACE
7. 5'RACE
8. Isolation of Plasmid
9. Competent cell preparation
10. Gene Cloning
11. End labelling of DNA
12. SDS and NATIVE PAGE
13. Western Blotting
14. EMSA (Electrophoretic Mobility Shift Assay)
15. Silver staining
16. Cell Culture
17. MTT assay
18. Lactate Dehydrogenase assay
19. Luciferace assay
20. Co-IP
21. Protein purification
22. Sub cellular fractionation
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Fluorescent activated cell sorting (FACS)
26. Basics of Bioinformatics
27. GST Pulldown assay
28. Fluorescence In situ Hybridization
29. Yeast Two Hybrid Assay
30. ELISA (Enzyme Linked Immuno Sorbent Assay)

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From the Publisher

A practical handbook detailing a range of methods and techniques central to the field of molecular biology