The HPLC Solvent Guide / Edition 2

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Overview

* Reviews the role of solvents in HPLC.

• Detailed and expanded presentation reviewing all commonly-used solvent classes and chemical and physical properties.

• Easy reference for solvent class, field of application, and specific analyte class.

• Each separation is described fully; listing sample matrix, analytes separated and quantified, chromatographic parameters used, and abbreviated results.

• Popular "how to" and "how not to" methodology.

• Tables feature physical, chemical, and chromatographic properties of individual solvent classes; safety and health parameters; availability in terms of supplier and performance specifications; and chemical structures of solvents.

• Focus is on practical applications and use of method optimization processes.

New to the 2nd edition:

• Detailed discussion of method optimization techniques (triangulation, window diagrams).

• The concept of method validation is presented, including functional definitions and general guidelines for validation protocols.

• Detailed discussion of system suitability parameters, what they are, and how they are used.

• Over 600 new abstracted articles reviewed, building on 1,100 articles in the 1st edition.

• A key area of increased focus is natural products/botanicals/herbals.

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Editorial Reviews

From the Publisher
"...a convenient handbook to anyone wishing to improve his of her ability to develop and implement successful HPLC applications..." (Analytical and Bioanalytical Chemistry, Vol 378(1), Jan 2004)

"...offers practical and operational information about solvents for use in high-pressure liquid chromatography." (Journal of the American Chemical Society, Vol. 124, No. 35, 2002)

"...describes all of the commonly used HLPC solvents." (SciTech Book News, Vol. 26, No. 2, June 2002)

"...a convenient handbook for anyone wishing to improve his or her ability to develop and implement successful HPLC applications." (Annals of Bioanalytical Chemistry, Vol. 378)

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Product Details

  • ISBN-13: 9780471411383
  • Publisher: Wiley
  • Publication date: 2/27/2002
  • Edition description: REV
  • Edition number: 2
  • Pages: 664
  • Product dimensions: 6.38 (w) x 9.74 (h) x 1.41 (d)

Meet the Author

Paul C. Sadek is President of Analytical Consulting Laboratories, a manufacturing and research facility located in Kentwood, Michigan. Previously, he spent more than fifteen years in academia and industry, including many years in research and development with Burdick Jackson, the world's premier solvent manufacturer. Dr. Sadek also wrote Troubleshooting HPLC Systems: A Bench Manual.

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Table of Contents

Preface.

Introduction.

Abbreviations, Acronyms, and Symbols.

Chapter 1: Physical and Chemical Solvent Properties.

Chapter 2: Method Optimization.

Chapter 3: Method Validation and Ongoing Performance Evaluation.

Chapter 4: Alcohols.

Chapter 5: Alkanes and Alkyl Aromatics.

Chapter 6: Chlorinated Alkanes and Chlorinated Benzenes.

Chapter 7: Ethers.

Chapter 8: Keotones and Esters.

Chapter 9: Nitriles and Nitrogenous Solvents.

Chapter 10: Water, Dimethyl Sulfoxide, and Common Acidic Modifiers.

References.

Index.

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