Theory and Practice of Histological Techniques / Edition 6

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Overview

Here's the latest edition of the leading reference work on histological techniques. This book is an essential and invaluable resource no matter what part you play in histological preparations and applications, whether you're a student or a highly experienced laboratory professional. This is a one stop reference book that will help you at the beginning of your career and which will remain valuable to you, even as you gain experience in daily practice.

• Contributions from almost 30 expert contributors involved in teaching, research and practice provide a well-rounded perspective.

• Extensive summary tables, charts and boxes make reference easy

• Color photomicrographs provide visual guidance on the latest techniques.

• Expanded coverage of immunohistochemistry helps you make optimal use of this crucial approach.

• New coverage of molecular genetics as it applies to histopathological diagnoses.

• 5 brand new chapters on Ergonomics, Laser Capture, Tissue Microarray, GrossingTechniques, and Genetics keep you up to speed on the latest techniques.

• New material on Quality Assurance and Audit facilitates day-to-day laboratory management.

Techniques in neuropathology/connective tissues & stains/enzyme histochemistry/quantitation in histopathology.

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Editorial Reviews

From the Publisher
New Edition Edited Book Winner at The Royal Society of Medicine &The Society of Authors’ Medical Book Awards
“This book will be an invaluable adjunct to the histology biomedical scientist – from the student to the highly experienced. It provides in-depth information covering a full range of histological techniques from the basic sectioning to the advanced immunocytochemistry.”
Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Histology is an evolving science that is essential for the practice of modern pathology and basic research. Histologic methods reveal the organization of molecules and cells into complex tissues (both normal and pathologic). This practical handbook carefully describes theory and protocols for preparing cells and tissues for microscopic evaluation. Its 32 chapters cover topics ranging from principles of light microscopy to enzyme histochemistry and its diagnostic applications. The primary focus is on biochemistry, cell biology, and histology.
Purpose: According to the editors, this book is "designed as a comprehensive reference work for histopathology." They write: "the successful training of laboratory staff of all grades requires a thorough grounding in all aspects of histological techniques." The chapters are authored by expert contributors who delve into both theoretical and practical discussions.
Audience: The book is primarily designed for laboratory technicians in departments of laboratory medicine, pathology, anatomy, and cell biology. It designed for beginning students of histology, as wells as advanced professionals (clinical researchers, histologists and pathologists). Biomedical scientists interested in cell and developmental biology will also appreciate the wealth of practical information presented in this elegant, hardcover book.
Features: Each chapter begins with an overview of basic concepts, followed by formulas for mixing stains and dyes, step-by-step protocols, and beautiful examples of expected results. Each chapter lists key references and relevant web sites. Major headers and tables are color-coded, helping readers identify staining protocols of interest and distinguish ancillary data from stain formulas. Readers will find special stains for lipids, carbohydrates, nucleic acids, pigments and amyloid. Many chapters include tips for solving problems that may be encountered in the laboratory. The depth and breadth of topics covered in this reference book are impressive: from setting up your laboratory to personal ergonomics. The book includes an appendix that summarizes details regarding buffers, mounting media, and slide coatings.
Assessment: This comprehensive book explains the theory and practice of histology from A-to-Z. It is an excellent practical guide and reference manual. The emphasis on new staining techniques helps keep the field fresh and enhances the role of histology in the biomedical sciences. In addition to traditional dyes and tissue stains, current histologic techniques include immunohistochemistry, fluorescence in situ hybridization (FISH), and molecular pathology. The practice of pathology is increasingly relying on these advanced techniques for diagnosis and patient care. These new methodologies are fully addressed in this new edition. The text is well written and the quality of the full-color photomicrographs is outstanding. I have some criticism regarding methods for enzyme histochemistry and immunohistochemistry. Many common stains and techniques are only mentioned in passing. Suppose you order peroxidase-conjugated or alkaline phosphatase conjugated second antibodies to detect antigens in tissue sections. How would you proceed? ABC immunoperoxidase and PAP procedures are only mentioned in passing. I could not find a formula for a simple DAB staining solution. Similarly, suppose you wanted to detect membrane-associated alkaline phosphate on primordial germ cells. How would you fix the embryos to preserve this enzyme? Can you identify enzymes in situ and then perform two-color labeling with antibodies? Key procedures are occasionally hidden from view by the rich detail and comprehensive nature of this book. Despite these minor criticisms, this is an excellent, carefully edited laboratory handbook. It is a pleasure to read and it will be a welcome resource for hospital staff in departments of laboratory medicine and pathology. It also provides a goldmine of information for basic science researchers studying cellular and developmental biology.
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Product Details

  • ISBN-13: 9780443102790
  • Publisher: Elsevier Health Sciences
  • Publication date: 10/4/2007
  • Edition description: REV
  • Edition number: 6
  • Pages: 744
  • Product dimensions: 7.30 (w) x 9.80 (h) x 1.80 (d)

Meet the Author

Formerly Pathology Directorate and Business Manager, Queen's Medical Center, Nottingham, UK

Formerly Quality Assurance Co-ordinator, Cellular Pathology Services, Kaiser Permanente Regional Reference Laboratories, North Hollywood, CA, USA

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Table of Contents

Managing the Laboratory
Safety in the Laboratory
Light Microscopy
Fixation of Tissues
The Gross Room/Surgical Cutup
Tissue Processing
Microtomy: Paraffin and Frozen
How do Histological Stains Work?
The Hematoxylins and Eosin
Connective Tissues and Stains
Carbohydrates
Lipids
Proteins and Nucleic Acids
Pigments and MInerals
Amyloid
The Dispersed Neuroendocrine System, Cytoplasmic Granules, and other Organelles
Microorganisms
Bone
Techniques in Neuropathology
Enzyme Histochemistry and its Diagnostic Applications
Immunohistochemical Techniques
Immunohistochemistry Quality Control
Immunohistochemistry Applications in Pathology
Immunofluorescent Techniques
Tissue Microarray
Molecular Pathology—In Sits Hybridization
Genetic Testing: Utilization of Fluorescent In Situ Hybridization (FISH)
Laser Microdissection
Plastic Embedding for Light Microscopy
Electron Microscopy
Quantitative Data from Microscopic Specimens
Ergonomics
Appendices

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