Therapeutic Applications of Ribozymes / Edition 1

Therapeutic Applications of Ribozymes / Edition 1

by Kevin J. Scanlon
     
 

ISBN-10: 0896034771

ISBN-13: 9780896034778

Pub. Date: 06/23/1998

Publisher: Springer-Verlag New York, LLC

Expert laboratory scientists describe in detail their methodologies fo r constructing ribozymes designed to elucidate the role of specific ge nes as key routes to the development of novel therapies for a wide var iety of diseases. The authors review the many sites targeted with ribo zymes in various diseases and provide specific accounts of the practic al

Overview

Expert laboratory scientists describe in detail their methodologies fo r constructing ribozymes designed to elucidate the role of specific ge nes as key routes to the development of novel therapies for a wide var iety of diseases. The authors review the many sites targeted with ribo zymes in various diseases and provide specific accounts of the practic al techniques required for the proper use of ribozymes in these system s. Their cutting-edge protocols demonstrate how to achieve ribozyme ex pression in distinct cellular systems, the preparation and use of high -efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition. Therapeutic Applic ations of Ribozymes contains all the technical support needed to make ribozyme technology a widely used and significant methodology.

Product Details

ISBN-13:
9780896034778
Publisher:
Springer-Verlag New York, LLC
Publication date:
06/23/1998
Series:
Methods in Molecular Medicine Series, #11
Edition description:
1998
Pages:
480
Product dimensions:
6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Small Efficient Hammerhead Ribozymes
Philip Hendry, Trevor J. Lockett, and Maxine J. McCall

The Hairpin Ribozyme: Discovery and Development for Gene Therapy
Arnold Hampel

Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus
N. Kyle Tanner

The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme
Lun-Quan Sun, Wayne Gerlach, and Geoff Symonds

Methods for Treating HIV by Gene Therapy Using Ribozymes
Eric M. Poeschla, Mang Yu, Mark C. Leavitt, and Flossie Wong-Staal

An Expression Vector for Multiple Ribozymes
Jun Ohkawa, Yutaka Takebe, and Kazunari Taira

Viral RNA Targets for Ribozymes: Influenza A Virus
Gerd Hobom and Annette Menke

Hammerhead Ribozyme-Mediated Cleavage of Hepatitis B Virus RNA
Fritz von Weizsäcker, Hubert E. Blum, and Jack R. Wands

Bovine Leukemia Virus Ribozymes
Glenn H. Cantor

Use of Ribozymes to Specifically Alter the MDR-1 Phenotype
David Y. Bouffard

Adenoviral-Mediated Anti-H-ras Ribozyme for the Treatment of Bladder Cancer
Akira Irie, Jerry Tsai, and Mohammed Kashani-Sabet

Tissue-Specific Delivery of an Anti-H-ras Ribozyme Against Malignant Melanoma
Tsukasa Ohkawa and Mohammed Kashani-Sabet

Ribozymes as a Novel Approach for the Treatment of Human Pancreatic Carcinoma
Hiroshi Kijima, David Y. Bouffard, and Kevin J. Scanlon

K-ras Ribozyme for Lung Cancer
Alex W. Tong, Yu-An Zhang, John Nemunaitis,
and Gabriele Mues

Anti-c-erb-B-2 Ribozyme for Breast Cancer
Toshiya Suzuki, Lisa D. Curcio, Jerry Tsai,
and Mohammed Kashani-Sabet

Ribozymes: Applications in Cancer Metastasis Research
Gunhild M. Mælandsmo, Inge Kjønniksen, Eivind Hovig, Kevin J. Scanlon, and Øystein Fodstad

Preformed Ribozymes: Strategies for Design and In Vivo Applicationas Anticytokine Agents
Mouldy Sioud

Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells
Lance H. Leopold, Scott K. Shore, Ramana M. V. Reddy, and E. Premkumar Reddy

Ribozymes Targeted Against AML1/MTG8: Evaluation of Ribozyme Efficiency in a Cell-Free System and Transfection with Cationic Liposome
Hiromichi Matsushita, Hiroyuki Kobayashi, Masahiro Kizaki, and Yasuo Ikeda

Ribozyme Targeting of the Growth Factor Pleiotrophin
Frank Czubayko, Anke M. Schulte, and Anton Wellstein

Ribozyme-Mediated Inhibition of Cell Proliferation: A Model for Identifying and Refining a Therapeutic Ribozyme
Thale C. Jarvis, Dennis Macejak, and Larry Couture

Inhibition of bAPP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System
Marilyn Smedman and Robert B. Denman

Selective Modification of Fatty Acid Synthesis Pathway by Expression of an Acetyl-CoA Carboxylase-Specific Ribozyme Gene
Joohun Ha

In Vivo Knockout of a Tissue-Specific Gene by Synthetic Ribozymes
S. Petter Lyngstadaas, Brian S. Sproat, Martina Blaschke, Janos Ludwig, Thomas Rupp, and Hans P. Prydz

Ribozyme-Mediated Downregulation of Gene Expression in Transgenic Mice
Shimon Efrat

Targeted Cleavage of RNA Using External Guide Sequences and Eukaryotic RNase P
Yan Yuan and Fenyong Liu

Application of In Vitro Selection in Design of External Guide Sequences That Direct Efficient Cleavage of RNA by RNase P
Yan Yuan

A PCR-Based Approach to Hammerhead Ribozyme Synthesis
Katalin Karikó and Elliot S. Barnathan

Measurement of Ribozyme Activity Using RNase Protection
P. Kamath and E. J. Shillitoe

Index

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