Therapeutic Applications of Ribozymes / Edition 1

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Expert laboratory scientists describe in detail their methodologies fo r constructing ribozymes designed to elucidate the role of specific ge nes as key routes to the development of novel therapies for a wide var iety of diseases. The authors review the many sites targeted with ribo zymes in various diseases and provide specific accounts of the practic al techniques required for the proper use of ribozymes in these system s. Their cutting-edge protocols demonstrate how to achieve ribozyme ex pression in distinct cellular systems, the preparation and use of high -efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition. Therapeutic Applic ations of Ribozymes contains all the technical support needed to make ribozyme technology a widely used and significant methodology.

The book contains black-and-white illustrations.

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Editorial Reviews

From the Publisher
". . . an impressive collection of ribozyme applications. . .a useful investment for anyone who contemplates or is actively using ribozymes for target RNA inactivations."-Nature Medicine
Comprises 29 papers which provide an overview of the utility of ribozomes to selectively inhibit the expression of RNA. The authors review the many sites targeted with ribozymes in various diseases and provide specific accounts of the practical techniques required for the proper use of ribozymes in these systems. Specific topics include small efficient hammerhead ribozymes, discovery and development for gene therapy in the hairpin ribozyme, methods for treating HIV by gene therapy using ribozymes, in vivo knockout of a tissue-specific gene by synthetic ribozymes, and a PCR-based approach to hammerhead ribozyme synthesis. Also covered are potential problems and pitfalls in ribozyme biology and how to avoid them. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780896034778
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/23/1998
  • Series: Methods in Molecular Medicine Series , #11
  • Edition description: 1998
  • Edition number: 1
  • Pages: 480
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.06 (d)

Table of Contents

Small Efficient Hammerhead Ribozymes
Philip Hendry, Trevor J. Lockett, and Maxine J. McCall

The Hairpin Ribozyme: Discovery and Development for Gene Therapy
Arnold Hampel

Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus
N. Kyle Tanner

The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme
Lun-Quan Sun, Wayne Gerlach, and Geoff Symonds

Methods for Treating HIV by Gene Therapy Using Ribozymes
Eric M. Poeschla, Mang Yu, Mark C. Leavitt, and Flossie Wong-Staal

An Expression Vector for Multiple Ribozymes
Jun Ohkawa, Yutaka Takebe, and Kazunari Taira

Viral RNA Targets for Ribozymes: Influenza A Virus
Gerd Hobom and Annette Menke

Hammerhead Ribozyme-Mediated Cleavage of Hepatitis B Virus RNA
Fritz von Weizsäcker, Hubert E. Blum, and Jack R. Wands

Bovine Leukemia Virus Ribozymes
Glenn H. Cantor

Use of Ribozymes to Specifically Alter the MDR-1 Phenotype
David Y. Bouffard

Adenoviral-Mediated Anti-H-ras Ribozyme for the Treatment of Bladder Cancer
Akira Irie, Jerry Tsai, and Mohammed Kashani-Sabet

Tissue-Specific Delivery of an Anti-H-ras Ribozyme Against Malignant Melanoma
Tsukasa Ohkawa and Mohammed Kashani-Sabet

Ribozymes as a Novel Approach for the Treatment of Human Pancreatic Carcinoma
Hiroshi Kijima, David Y. Bouffard, and Kevin J. Scanlon

K-ras Ribozyme for Lung Cancer
Alex W. Tong, Yu-An Zhang, John Nemunaitis,
and Gabriele Mues

Anti-c-erb-B-2 Ribozyme for Breast Cancer
Toshiya Suzuki, Lisa D. Curcio, Jerry Tsai,
and Mohammed Kashani-Sabet

Ribozymes: Applications in Cancer Metastasis Research
Gunhild M. Mælandsmo, Inge Kjønniksen, Eivind Hovig, Kevin J. Scanlon, and Øystein Fodstad

Preformed Ribozymes: Strategies for Design and In Vivo Applicationas Anticytokine Agents
Mouldy Sioud

Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells
Lance H. Leopold, Scott K. Shore, Ramana M. V. Reddy, and E. Premkumar Reddy

Ribozymes Targeted Against AML1/MTG8: Evaluation of Ribozyme Efficiency in a Cell-Free System and Transfection with Cationic Liposome
Hiromichi Matsushita, Hiroyuki Kobayashi, Masahiro Kizaki, and Yasuo Ikeda

Ribozyme Targeting of the Growth Factor Pleiotrophin
Frank Czubayko, Anke M. Schulte, and Anton Wellstein

Ribozyme-Mediated Inhibition of Cell Proliferation: A Model for Identifying and Refining a Therapeutic Ribozyme
Thale C. Jarvis, Dennis Macejak, and Larry Couture

Inhibition of bAPP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System
Marilyn Smedman and Robert B. Denman

Selective Modification of Fatty Acid Synthesis Pathway by Expression of an Acetyl-CoA Carboxylase-Specific Ribozyme Gene
Joohun Ha

In Vivo Knockout of a Tissue-Specific Gene by Synthetic Ribozymes
S. Petter Lyngstadaas, Brian S. Sproat, Martina Blaschke, Janos Ludwig, Thomas Rupp, and Hans P. Prydz

Ribozyme-Mediated Downregulation of Gene Expression in Transgenic Mice
Shimon Efrat

Targeted Cleavage of RNA Using External Guide Sequences and Eukaryotic RNase P
Yan Yuan and Fenyong Liu

Application of In Vitro Selection in Design of External Guide Sequences That Direct Efficient Cleavage of RNA by RNase P
Yan Yuan

A PCR-Based Approach to Hammerhead Ribozyme Synthesis
Katalin Karikó and Elliot S. Barnathan

Measurement of Ribozyme Activity Using RNase Protection
P. Kamath and E. J. Shillitoe


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